FD6601 - BIOCHEMICAL ENGINEERING (Syllabus) 2013-regulation Anna University

FD6601 - BIOCHEMICAL ENGINEERING (Syllabus) 2013-regulation Anna University

FD6601

BIOCHEMICAL ENGINEERING

 LPTC

3003

UNIT I

INTRODUCTION TO ENZYMES

9

Classification of enzymes. Mechanisms of enzyme action; concept of active site and energetics of enzyme substrate complex formation; specificity of enzyme action; principles of catalysis – collision theory, transition state theory; role of entropy in catalysis.

UNIT II

KINETICS OF ENZYME ACTION

9

Kinetics of single substrate reactions; estimation of Michelis – Menten parameters, multisubstrate reactions- mechanisms and kinetics; turnover number; types of inhibition & models –substrate, product. Allosteric regulation of enzymes, Monod changeux wyman model, ph and temperature effect on enzymes & deactivation kinetics.


UNIT III

ENZYME IMMOBILIZATION

6

Physical and chemical techniques for enzyme immobilization – adsorption, matrix entrapment, encapsulation, cross-linking, covalent binding etc., - examples, advantages and disadvantages.

UNIT IV

OVERVIEW OF FERMENTATION PROCESSES

9

Overview of fermentation industry, general requirements of fermentation processes, basic configuration of fermentor and ancillaries, main parameters to be monitored and controlled in fermentation processes.

UNIT V

RAW MATERIALS AND MEDIA DESIGN FOR FERMENTATION PROCESS

12

Criteria for good medium, medium requirements for fermentation processes, carbon, nitrogen, minerals, vitamins and other complex nutrients, oxygen requirements, medium formulation of optimal growth and product formation, examples of simple and complex media, design of various commercial media for industrial fermentations – medium optimization methods

TOTAL : 45 PERIODS

TEXT BOOKS:
1. Bailey, J.E. and Ollis, D.F. “Biochemical Engineering Fundamentals”, 2nd Edition, McGraw-Hill, 1986.
2. Blanch, H.W. and D.S. Clark “Biochemical Engineering”, Marcal Dekker, Inc., 1997.
3. Lee, James M. “Biochemical Engineering”, Prentice – Hall, 1992.

REFERENCES
1. Palmer, Trevor “Enzymes : Biochemistry, Biotechnology, Clinical Chemistry”, Affiliated East-West Press Pvt. Ltd., 2004.
2. Stanbury, P.F., A. Whitaker and S.J. Hall “Principles of Fermentation Technology”, 2nd Edition, Butterworth – Heinemann (an imprint of Elsevier), 1995.
3. Wiseman, Alan “Handbook of Enzyme Biotechnology”, 3rd Edition, Ellis Harwood Publications, 1999.
4. Hartmeier, Winfried “Immobilized Biocatalysts : An Introduction”, Springer – Verlag, 1986

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