FD6701 - GENETIC ENGINEERING AND GENETICALLY MODIFIED FOOD (Syllabus) 2013-regulation Anna University

FD6701 - GENETIC ENGINEERING AND GENETICALLY MODIFIED FOOD (Syllabus) 2013-regulation Anna University

FD6701

GENETIC ENGINEERING AND GENETICALLY MODIFIED FOOD

 LPTC
3003

UNIT I

BASICS OF RECOMBINANT DNA TECHNOLOGY

9
Manipulation of DNA and RNA – Restriction and Modification enzymes, Design of linkers and adaptors. Characteristics of cloning and expression vectors based on plasmid and bacteriophage, Vectors for insect, yeast and mammalian system, Prokaryotic and eukaryotic host systems, Introduction of recombinant DNA in to host cells and selection methods.

UNIT II

DNA LIBRARIES

9
Construction of genomic and cDNA libraries, Artificial chromosomes – BACs and YACs, Screening of DNA libraries using nucleic acid probes and antisera.


UNIT III

SEQUENCING AND AMPLIFICATION OF DNA

9
Maxam Gilbert’s and Sanger’s methods of DNA sequencing. Inverse PCR, Nested PCR, AFLP-PCR, Allele specific PCR, Assembly PCR, Asymmetric PCR, Hot start PCR, inverse PCR, Colony PCR, single cell PCR, Real-time PCR/qPCR – SYBR green assay, Taqman assay, Molecular beacons. Site directed mutagenesis.

UNIT IV

TRANSGENIC TECHNOLOGY

9
DNA microinjection, Retroviral vectors, Transgenic animals – Knock in and knock out animals, Transgenic plants – Ti plasmid.

UNIT V

APPLICATIONS OF RDNA TECHNOLOGY IN FOODS (Remove company names)

9
Genetically engineered proteins: Bovine Somatotropin in Milk; Genetically engineered bacteria:Chymosin Lite beer; Tryptophan; Transgenic plants: Calgene Flavr SavrTM tomato,MonsantoRound-UpTM Ready, Ciba Geigy BastaTM resistant crops; Edible vaccines:Cholera vaccine in potatoes; Transgenic Fish: Atlantic salmon.

TOTAL : 45 PERIODS

TEXT BOOKS:
1. Rees, Andy “Genetically Modifies Food: A Short Guide for the Confused”. Pluto Press, 2006.
2. Ahmed, Farid E. “Testing of Genetically Modified Organisms in Food”. Food Products Press, 2004.

REFERENCE
1. Halford, Nigel G. “Genetically Modified Crops”. Imperial College Press, 2003.
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