BT8005 - ANIMAL BIOTECHNOLOGY (Syllabus) 2017-regulation Anna University

BT8005 - ANIMAL BIOTECHNOLOGY (Syllabus) 2017-regulation Anna University

BT8005

ANIMAL BIOTECHNOLOGY

 LPTC
3003

OBJECTIVES:
To provide the fundamentals of animal cell culture, details of the diseases and therapy • To offer the knowledge about the micromanipulation and transgenic animals

UNIT I

ANIMAL CELL CULTURE

9
Introduction to basic tissue culture techniques; chemically defined and serum free media; animal cell cultures, their maintenance and preservation; various types of cultures suspension cultures, continuous flow cultures, immobilized cultures; somatic cell fusion; cell cultures as a source of valuable products; organ cultures.

UNIT II

ANIMAL DISEASES AND THEIR DIAGNOSIS

9
Bacterial and viral diseases in animals; monoclonal antibodies and their use in diagnosis; molecular diagnostic techniques like PCR, in-situ hybridization; northern and southern blotting; RFLP.


UNIT III

THERAPY OF ANIMAL DISEASES

9
Recombinant cytokines and their use in the treatment of animal infections; monoclonal antibodies in therapy; vaccines and their applications in animal infections; gene therapy for animal diseases.

UNIT IV

MICROMANIPULATION OF EMBRYO’S

9
What is micromanipulation technology; equipments used in micromanipulation; enrichment of x and y bearing sperms from semen samples of animals; artificial insemination and germ cell manipulations; in vitro fertilization and embryo transfer; micromanipulation technology and breeding of farm animals.

UNIT V

TRANSGENIC ANIMALS

9
Concepts of transgenic animal technology; strategies for the production of transgenic animals and their importance in biotechnology; stem cell cultures in the production of transgenic animals.

TOTAL : 45 PERIODS

OUTCOMES: Upon completion of this subject the student will be able to
• Understand the animal cell culture, animal diseases and its diagnosis
• Gain the knowledge for therapy of animal infections
• Know the concepts of micromanipulation technology and transgenic animal technology
• Use the knowledge gained in this section to apply in the field of clinical research

TEXT BOOKS:
1. Ranga M.M. Animal Biotechnology. Agrobios India Limited, 2002
2. Ramadass P, Meera Rani S. Text Book Of Animal Biotechnology. Akshara Printers, 1997.

REFERENCE:
1. Masters J.R.W. Animal Cell Culture: Practical Approach. Oxford University Press.2000
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