BT8008 - MOLECULAR PATHOGENESIS OF INFECTIOUS DISEASES (Syllabus) 2017-regulation Anna University

BT8008 - MOLECULAR PATHOGENESIS OF INFECTIOUS DISEASES (Syllabus) 2017-regulation Anna University

BT8008	MOLECULAR PATHOGENESIS OF INFECTIOUS DISEASES	LPTC

3003

OBJECTIVES:

To enable the students • To understand about the microbial toxins and modern molecular pathogenesis • To know about the host pathogen interaction and identifying virulence factors • To control pathogens by modern approaches.

UNIT I	OVERVIEW	5

Historical perspective - discovery of microscope, Louis Pasteur’s contributions, Robert Koch’s postulates, early discoveries of microbial toxins, toxic assays, vaccines, antibiotics and birth of molecular genetics and modern molecular pathogenesis studies, Various pathogen types and modes of entry.

UNIT II	HOST-DEFENSE AGAINST PATHOGENS AND PATHOGENIC STRATEGIES	8

Attributes & components of microbial pathogenesis, Host defense: skin, mucosa, cilia, secretions, physical movements, limitation of free iron, antimicrobial compounds, mechanism of killing by humoral and cellular defense mechanisms, complements, inflammation process, general disease symptoms, Pathogenic adaptations to overcome the above defenses.

UNIT III	MOLECULAR PATHOGENESIS (WITH SPECIFIC EXAMPLES)	16

Virulence, virulence factors, virulence- associated factors and virulence lifestyle factors, molecular genetics and gene regulation in virulence of pathogens, Vibrio Cholerae: Cholera toxin, co- regulated pili, filamentous phage, survival E.coli pathogens: Enterotoxigenic E.coli (ETEC), labile & stable toxins, Entero- pathogenic E.coli (EPEC), type III secretion, cytoskeletal changes, intimate attachment; Enterohaemerrohogic E.coli (EHEC), mechanism of bloody diarrhoea and Hemolytic Uremic Syndrome, Enteroaggregative E.coli (EAEC). Shigella: Entry, macrophage apoptosis, induction of macropinocytosis, uptake by epithelial cells, intracellular spread, inflammatory response, tissue damage Plasmodium: Life cycle, erythrocyte stages, transport mechanism and processes to support the rapidly growing schizont, parasitiparous vacuoles, and knob protein transport, Antimalarials based on transport processes. Influenza virus: Intracellular stages, Neuraminidase & Haemagglutinin in entry, M1 & M2 proteins in assembly and disassembly, action of amantidine.

UNIT IV	EXPERIMENTAL STUDIES ON HOST-PATHOGEN INTERACTIONS	8

Virulence assays: adherence, invasion, cytopathic, cytotoxic effects. Criteria & tests in identifying virulence factors, attenuated mutants, molecular characterization of virulence factors, signal transduction & host responses

UNIT V	APPROACHES TO CONTROL PATHOGENS	8

Classical approaches based on serotyping. Modern diagnosis based on highly conserved virulence factors, immuno & DNA-based techniques. New therapeutic strategies based on recent findings on molecular pathogenesis of a variety of pathogens, Vaccines - DNA, subunit and cocktail vaccines.

TOTAL : 45 PERIODS

OUTCOMES: Upon completion of this course, the student will be able to understand the

• Host pathogen interactions at the level of cellular and molecular networks.
• Diagnosis of diseases through the examination of molecules.
• Modern therapeutic strategies on various pathogens.

REFERENCES:

1. Iglewski B.H and Clark V.L “Molecular basis of Bacterial Pathogenesis“, Academic Press, 1990.
2. Peter Williams, Julian Ketley & George Salmond, “Methods in Microbiology: Bacterial Pathogenesis, Vol. 27”, Academic Press, 1998.
3. Recent reviews in Infect. Immun., Mol. Microbiol., Biochem. J., EMBO etc
4. Nester, Anderson, Roberts, Pearsall, Nester, “Microbiology: A Human Perspective”, McGraw Hill, 3rd Edition, 2001.
5. Eduardo A. Groisman, Principles of Bacterial Pathogenesis, Academic Press, 2001.