BT8016 - GENOMICS AND PROTEOMICS (Syllabus) 2017-regulation Anna University

BT8016 - GENOMICS AND PROTEOMICS (Syllabus) 2017-regulation Anna University

BT8016	GENOMICS AND PROTEOMICS	LPTC

3003

OBJECTIVES:

• To provide the students a broader knowledge on the structure and function of genomes, the technologies developed for genomics, functional genomics and proteomics.

UNIT I	INTRODUCTION	9

Introduction to genome, transcriptome, and proteome; Overview of genomes of bacteria, archae, and eukaryote; Genomes of organelles.

UNIT II	GENOME MAPPING AND SEQUENCING	9

Genetic and physical mapping, Linkage analysis, RFLP, SNP, SSLP, Restriction mapping, STS mapping, FISH, Top-down and bottom-up sequencing strategies, Whole genome sequencing, Gap closure, Pooling strategies.

UNIT III	FUNCTIONAL GENOMICS	9

Genome annotation, ORF and functional prediction, Gene finding, Substractive DNA library screening, Differential display and Representational difference analysis, SAGE,TOGA, Introduction to DNA microarray.

UNIT IV	TECHNIQUES IN PROTEOMICS	9

In-vitro and in vivo-labeling of proteins, One and two-dimensional gel electrophoresis, Detection of proteins on SDS gels, Protein cleavage, Edman protein microsequencing, Mass spectrometry- principles of MALDI-TOF, Peptide mass fingerprinting.

UNIT V	PROTEIN PROFILING	9

Large-scale protein profiling using proteomics, Post-translational modifications, Phosphoprotein and glycoprotein analyses; Analysis of protein-protein interactions, Protein microarrays.

TOTAL : 45 PERIODS

OUTCOMES:

• The students would have gained a better understanding of the organization of genomes in multiple levels of taxa, and the methodologies and approaches used for the study of structural and functional genomics. The students would have also acquired knowledge on various genome mapping and sequencing methods, genomic markers, microarray technology and methods for proteomics.

TEXT BOOKS:

1. Suhai, Sandor “Genomics and Proteomics: Functional and Computational Aspects”. Springer, 2000
2. Pennington, S.R. and M.J. Dunn “Proteomics: From Protein Sequence to Function”. VivaBooks Pvt. Ltd., 2002.
3. O’Connor, C.D. and B.D.Hames. “ Proteomics”. Scion Publishing, 2008.
4. Primrose, S.B. and Twyman. “Principles of Genome Analysis and Genomics”. 7th Edition, Blackwell Publishing, 2006

REFERENCES:

1. Cantor, Charles R. and Cassandra L. Smith. “Genomics: The Science and Technology Behind the Human Genome Project”. John Wiley & Sons, 1999.
2. Liebler, R.C. “Introduction to Proteomics”. Humana Press, 2002.
3. Hunt, Stephen P. and Frederick J. Livesey. “Functional Genomics”. Oxford University Press,2000.
4. Conard, Edward. “Genomics”. Apple Academics, 2010