BT8026 - STEM CELL TECHNOLOGY (Syllabus) 2017-regulation Anna University

BT8026 - STEM CELL TECHNOLOGY (Syllabus) 2017-regulation Anna University

BT8026

STEM CELL TECHNOLOGY

 LPTC
3003

OBJECTIVES:
• The course objectives are imparting the basic knowledge of students about stem cell, culturing and its clinical applications.

UNIT I

STEM CELLS AND TYPES

9
Stem cells: Definition, Classification, Sources and Properties –Types of stem cells: methods of isolation, study of stem cells and their viability IPSC, embryonic stem cells, cancer stem cells. – Preservations of Stem cell. Embryonic stem cell: Isolation, Culturing, Differentiation, Properties – Adult stem cell: Isolation, Culturing, Differentiation, Trans-differentiation, Plasticity, and Properties

UNIT II

STEM CELLS IN PLANTS AND ANIMALS

9
Stem cell and founder zones in plants –particulary their roots – stem cells of shoot meristems of higher plants. Skeletal muscle stem cell – Mammary stem cells – intestinal stem cells – keratinocyte stem cells of cornea – skin and hair follicles –tumour stem cells.


UNIT III

STEM CELLS DIFFERENTIATION

9
Factors influencing proliferation, physical, chemical and molecular methods for differentiation of stem cells – hormonal role in differentiation.

UNIT IV

REGENERATION AND EXPERIMENTAL METHODS

9
Germ cells, hematopoietic organs, and kidney, cord blood transplantation, donor selection, HLA matching, patient selection, peripheral blood and bone marrow transplantation, - Stem cell Techniques: fluorescence activated cell sorting (FACS), time lapse video, green fluorescent protein tagging

UNIT V

APPLICATION AND ETHICAL ISSUES

9
Stem cell Therapy for neurodegenerative diseases, spinal cord injury, heart disease, diabetes, burns, skin ulcers, muscular dystrophy and orthopaedic applications. Stem cell policy and ethics, stem cell research: Hype, hope and controversy.

TEXT BOOKS:
1. Stem cells by C.S Potten., Elsevier, 2006.
2. Essentials of Stem Cell Biology by Robert Lanza., fourth edition. Elsevier 2014.

REFERENCES
1. Stem cell biology and Gene Therapy by Peter Quesenberry., First Edition, Wiley-Liss, 1998.
2. Embryonic Stem cells – Protocols by KursadTurksen., Second Edition Humana Press, 2002.
3. Stem Cells: From Bench to Bedside by AriffBongso, EngHinLee., World Scientific Publishing Company, 2005.
4. Stem cells in clinic and Research by Ali Gholamrezanezhad., Intech, 2013
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