BT8403 - ENZYME TECHNOLOGY AND BIO-TRANSFORMATIONS (Syllabus) 2017-regulation Anna University

BT8403 - ENZYME TECHNOLOGY AND BIO-TRANSFORMATIONS (Syllabus) 2017-regulation Anna University

BT8403

ENZYME TECHNOLOGY AND BIO-TRANSFORMATIONS

 LPTC

3003

OBJECTIVES: To enable the students
• To learn enzyme reactions and its characteristics along with the production and purification process
• To give the student a basic knowledge concerning biotransformation reactions with the usage of enzymes

UNIT I

INTRODUCTION TO ENZYMES

9

Classification of enzymes. Mechanisms of enzyme action; concept of active site and energetics of enzyme substrate complex formation; specificity of enzyme action; principles of catalysis – collision theory, transition state theory; role of entropy in catalysis.

UNIT II

KINETICS OF ENZYME ACTION

9

Kinetics of single substrate reactions; estimation of Michelis – Menten parameters, multisubstrate reactions - mechanisms and kinetics; turnover number; types of inhibition & models –substrate, product. Allosteric regulation of enzymes, Monod Changeux Wyman model, pH and temperature effect on enzymes & deactivation kinetics.


UNIT III

ENZYME IMMOBILIZATION AND BIOSENSORS

9

Physical and chemical techniques for enzyme immobilization – adsorption, matrix entrapment, encapsulation, cross-linking, covalent binding etc., - examples, advantages and disadvantages, design of enzyme electrodes and their application as biosensors in industry, healthcare and environment.

UNIT IV

PURIFICATION AND CHARACTERIZATION OF ENZYMES FROM NATURAL SOURCES

9

Production and purification of crude enzyme extracts from plant, animal and microbial sources; methods of characterization of enzymes; development of enzymatic assays

UNIT V

BIOTRANSFORMATION APPLICATIONS OF ENZYMES

9

Hydrolytic- Ester bond, Amide, Epoxides, Nitriles, Reduction reactions –aldehydes, Ketones, C=C, Oxidation reactions – Alkanes, Aromatic, Baeyer-Villiger, Enzymes in organic synthesis – esters, amide, peptide , Modified and Artificial Enzymes , Catalytic antibodies

TOTAL : 45 PERIODS

OUTCOMES:
• The knowledge on enzyme and enzyme reactions will be the key step in to proceed towards various concepts in biotechnology.
• The theoretical and practical aspects of kinetics will provide the importance and utility of enzyme kinetics towards research.
• The process of immobilization has been increased steadily in food, pharmaceutical and chemical industries and thus this study will provide simple and easy method of implementation.
• Ideas on Processing, Production and Purification of enzymes at an industrial scale will be helpful to work technologically.

TEXT BOOKS:
1. Trevor Palmer , Enzymes IInd Horwood Publishing Ltd
2. Faber K , Biotransformations in Organic Chemistry, IV edition , Springer

REFERENCES:
1. Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering, Marcel Dekker, Inc.
2. James M. Lee, Biochemical Engineering, PHI, USA.
3. James. E. Bailey & David F. Ollis, Biochemical Engineering Fundamentals, McGraw Hill.
4. Wiseman, Enzyme Biotechnology, Ellis Horwood Pub.

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