BT8591 - BIOPROCESS ENGINEERING (Syllabus) 2017-regulation Anna University

BT8591 - BIOPROCESS ENGINEERING (Syllabus) 2017-regulation Anna University

BT8591	BIOPROCESS ENGINEERING	LPTC

3003

OBJECTIVES:

• To provide the students with the basics of bioreactor engineering.
• To develop bioengineering skills for the production of biochemical product using integrated biochemical processes.

UNIT I	CONFIGURATION OF BIOREACTORS	9

Ideal reactors and its characteristicsFed batch cultivation, Cell recycle cultivation, Cell recycle cultivation in waste water treatment, two stage cultivation Packed bed reactor, airlift reactor, introduction to fluidized bed reactor bubble column reactors

UNIT II	BIOREACTOR SCALE – UP	9

Regime analysis of bioreactor processes, oxygen mass transfer in bioreactors – microbial oxygen demands; methods for the determination of mass transfer coefficients; mass transfer correlations. Scale up criteria for bioreactors based on oxygen transfer, power consumption and impeller tip speed.

UNIT III	BIOREACTOR CONSIDERATION IN ENZYME SYSTEMS	9

Analysis of film and pore diffusion effects on kinetics of immobilized enzyme reactions; formulation of dimensionless groups and calculation of effectiveness factors. Design of immobilized enzyme reactors – packed bed, fluidized bed and membrane reactors

UNIT IV	MODELLING AND SIMULATION OF BIOPROCESSES	9

Study of structured models for analysis of various bioprocess – compartmental models, models of cellular energetics and metabolism, single cell models, plasmid replication and plasmid stability model. Dynamic simulation of batch, fed batch, steady and transient culture metabolism.

UNIT V	RECOMBINANT CELL CULTIVATION	9

Different host vector system for recombinant cell cultivation strategies and advantages. E.coli, yeast Pichia pastoris / Saccharomyces cereviseae, Animal cell cultivation, plant cell cultivation, Insect cell cultivation. High cell density cultivation, process strategies, reactor considerations in the above system

TOTAL : 45 PERIODS

OUTCOMES: Upon completion of Bioprocess Engineering course graduates will be able to

• Select appropriate bioreactor configurations and operation modes based upon the nature of bioproducts and cell lines and other process criteria.
• Apply modeling and simulation of bioprocesses so as to reduce costs and to enhance the quality of products and systems.
• Plan a research career or to work in the biotechnology industry with strong foundation about bioreactor design and scale-up.
• Integrate research lab and Industry; identify problems and seek practical solutions for large scale implementation of Biotechnology.

TEXT BOOKS:

1. Michael L. Shuler and Fikret Kargi, Bioprocess Engineering, Basic Concept, 2nd Edition Prentice Hall PTR, 2002.
2. Pauline Doran, Bioprocess Engineering Calculation, Blackwell Scientific Publications

REFERENCES:

1. Anton Moser, “Bioprocess Technology, Kinetics and Reactors”, , Springer Verlag.
2. James E. Bailey & David F. Ollis, Biochemical Engineering Fundamentals, McGraw Hill.
3. James M. Lee, Biochemical Engineering, PHI, USA.
4. Atkinson, Handbook of Bioreactors,Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering, Marcel Decker Inc.
5. Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering, Marcel Dekker, Inc