BT6013 - BIOCONJUGATE TECHNOLOGY AND APPLICATIONS (Syllabus) 2013-regulation Anna University

BT6013 - BIOCONJUGATE TECHNOLOGY AND APPLICATIONS (Syllabus) 2013-regulation Anna University

BT6013

BIOCONJUGATE TECHNOLOGY AND APPLICATIONS

 LPTC

3003

OBJECTIVES: To enable the students
• To understand the concepts of bioconjugate technology and its applications.
• To understand the chemical properties of bioconjugates
• To study the importance/role of biological products in body functions.

UNIT I

FUNCTIONAL TARGETS

9

Modification of Amino Acids, Peptides and Proteins– Modification of sugars, polysaccharides and glycoconjugates – modification of nucleic acids and oligonucleotides.

UNIT II

CHEMISTRY OF ACTIVE GROUPS

9

Amine reactive chemical reactions – Thiol reactive chemical reactions – carboxylate reactive chemical reactions – hydroxyl reactive chemical reactions – aldehyde and ketone reactive chemical reactions – Photoreactive chemical reactions.


UNIT III

BIOCONJUGATE REAGENTS

9

Zero length cross linkers – Homobifunctional cross linkers – Heterobifunctional cross linkers – Trifunctional cross linkers – Cleavable reagent systems – tags and probes.

UNIT IV

ENZYME AND NUCLEIC ACID MODIFICATION AND CONJUGATION

9

Properties of common enzymes – Activated enzymes for conjugation – biotinylated enzymes – chemical modification of nucleic acids – biotin labeling of DNA- enzyme conjugation to DNA – Fluorescent of DNA.

UNIT V

BIOCONJUGATE APLICATIONS

9

Preparation of Hapten-carrier Immunogen conjugates - antibody modification and conjugation – immunotoxin conjugation techniques – liposome conjugated and derivatives- Colloidal – gold-labeled proteins – modification with synthetic polymers.

TOTAL : 45 PERIODS

OUTCOMES: The student will be able to
• Understand the characters and functions of bioconjugate materials.
• Understand the various chemical reactions involved in the modifications of bioconjugates.
• Understand the major applications of bioconjugates.

TEXT BOOKS:
1. Bioconjugate Techniques, G.T. Hermanson, Academic Press, 1999.

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