BT6201 - BIOCHEMISTRY (Syllabus) 2013-regulation Anna University

BT6201 - BIOCHEMISTRY (Syllabus) 2013-regulation Anna University

BT6201	BIOCHEMISTRY	LPTC

3003

OBJECTIVES:

• To develop understanding and provide scientific basics of the life processes at the molecular level and explain the structure, function and inter-relationships of bio- molecules and their deviation from normal and their consequences for interpreting and solving clinical problems.

UNIT I	BIOCHEMICAL ORGANIZATION AND BIOENERGETICS	10

Scope of clinical biochemistry, component of the cell, structure and biochemical functions, membrane structure and functions, transport through biological cell membrane, the concept of free energy, determination of change in free energy from equilibrium constant and reduction potential, bioenergetics and biological oxidation – general concept of oxidation and reduction, electron transport chain, oxidative phosphorylation, uncouplers and theories of biological oxidation and oxidative phosphorylation

UNIT II	BIOMOLECULES	12

Carbohydrates – classification, properties.starch, glycogen, dextrin, inulin, cellulose, metabolism of carbohydrates – gluconeogenesis, glycogenolysis, glycolysis. citric acid cycle and its biological significance, role of sugar in nucleotide biosynthesis and pentose phosphate pathway. Lipids – Classification, properties. sterols, essential fatty acids, eicosanoids, phospholipids, sphingolipids, metabolism of lipids, oxidation of fatty acids, α,β - oxidation and biosynthesis of ketone bodies, cholesterol, porphyrin biosynthesis, metabolism of bile pigments. Proteins and amino acids – Classification, properties, biosynthesis of amino acids and proteins, essential amino acids, metabolism of amino acids and proteins, Nitrogen balance. Nucleic acids –genetic code, nucleic acids, and structure of DNA and RNA, purine biosynthesis and pyrimidine biosynthesis.

UNIT III	BIOENERGETICS	5

High energy compounds, electronegative potential of compounds , respiratory chain, ATP cycle , Calculation of ATP during oxidation of glucose and fatty acids.

UNIT IV	MACROMOLECULES, VITAMINS, HORMONES, ENZYMES	10

Physical and chemical properties, structure of haemoglobin, immunoglobulins and nucleoprotein, classification and their properties, occurrence, functions, requirements, deficiency manifestations and role of vitamins as coenzyme, chemical nature and properties, hormones, Nomenclature, enzyme kinetics, classification and their properties, mechanism of action, enzyme induction and inhibition, coenzyme significance and enzymes of clinical importance

UNIT V	BIOCHEMISTRY OF CLINICAL DISEASES	8

Diabetes mellitus, atherosclerosis, fatty liver, and obesity, hormonal disorders, aging, inborn errors of metabolism organ function tests

TOTAL : 45 PERIODS

TEXT BOOKS:

1. Lehninger A.L., Nelson D.L. and Cox M.M. Principles of Biochemistry. CBS publishers and distributers
2. Murray R.K., Granner D.K., Mayes P.A. and Rodwell V.W. Harpers Biochemistry. Appleton and Lange ,Stanford ,Conneticut.
3. Thomas M. Devlin.Textbook of Biochemistry with clinical correlations. Wiley Liss Publishers

REFERENCES:

1. Burtis & Ashwood W.B. Tietz Textbook of Clinical chemistry. Saunders Company
2. Lubert Stryer W.H. Biochemistry. Freeman and company, New york.
3. Donald Voet & Judith G. Voet. Biochemistry. John Wiley and Sons ,Inc.
4. Rama Rao Textbook of Biochemistry.
5. Deb. Textbook of Biochemistry.