PY6004 - GENETIC ENGINEERING AND PHARMACOGENOMICS (Syllabus) 2013-regulation Anna University

PY6004 - GENETIC ENGINEERING AND PHARMACOGENOMICS (Syllabus) 2013-regulation Anna University

PY6004

GENETIC ENGINEERING AND PHARMACOGENOMICS

 LPTC
3003

OBJECTIVES:
To enable the student to know about genetic engineering and pharmacogenomics principles and application in human genome associated diseases. To provide the knowledge about the influence of genetic variation on drug response.

UNIT I

BASICS OF RECOMBINANT DNA TECHNOLOGY

9
Manipulation of DNA and RNA – Restriction and Modification enzymes, Design of linkers and adaptors. Characteristics of cloning and expression vectors based on plasmid and bacteriophage, Vectors for insect, yeast and mammalian system, Prokaryotic and eukaryotic host systems, Introduction of recombinant DNA in to host cells and selection methods.

UNIT II

DNA LIBRARIES

9
Construction of genomic and cDNA libraries, Artificial chromosomes – BACs and YACs, Chromosomal walking, Screening of DNA libraries using nucleic acid probes and antisera.


UNIT III

SEQUENCING AND AMPLIFICATION OF DNA

9
Maxim Gilbert’s and Sanger’s methods of DNA sequencing. PCR: Inverse, Nested, AFLP-, Allele specific, Assembly, Asymmetric, Hot start, inverse, Colony, single cell, Real-time PCR/qPCR – SYBR green assay, Taqman assay, Molecular beacons. Site directed mutagenesis.

UNIT IV

HUMAN GENOME AND ASSOCIATION STUDIES IN PHARMACOGENOMICS

9
Expressed sequence Tags (EST) and computational biology, Microbial genomics, computational analysis of whole genomes, computational genome analysis, Genomic differences that affect the outcome of host pathogen interactions: A template for the future of whole genome-based pharmacological science, Viability and ADR in drug response: contribution of genetic factor, Multiple inherited genetic factors influence the outcome of drug treatments, Plasma binding proteins, Drug targets.

UNIT V

PHARMACOGENOMICS AND DRUG DESIGN WITH CASE STUDIES

9
Understanding biology and diseases,Target identification and validation, Drug candidate identification and optimization incorporation of pharmacogenomics principles, cases studies for CVS, CNS, immunomodulators, antioxidants etc.

TOTAL : 45 PERIODS

OUTCOMES: The student will be able to
• Understand the influence of genetic variation on drug response in patients by correlating gene expression or single-nucleotide polymorphisms with a drug's efficacy or toxicity.
• Achieve an overview of advanced principles and techniques of human genetic engineering.

TEXT BOOKS:
1. Primrose, S. Twyman, R. “Principles of Gene Manipulation and Genomics” 7th Edition, Blackwell Publishing, 2006.
2. Chakraborty, Chiranjib and Atanu Bhatta Charya “Pharmacogenomics : An Approach to New Drug Development”, Biotech Books, 2004.

REFERENCES:
1. Brown, T.A. “Gene Cloning & DNA Analysis : An Introduction”, 5th Edition, Blackwell Publishing, 2006.
2. Glick, B.R. and J.J. Pasternak “Molecular Biotechnology : Principles and Applications of Recombinant DNA”, 3rd Edition, ASM, 2003.
3. Licinio, Julio and Ma-Liwong “Pharmacogenomics : The Search for Individualized Therapies”, Wiley – VCH, 2002.
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