PY6012 - DOWNSTREAM PROCESSING (Syllabus) 2013-regulation Anna University

PY6012 - DOWNSTREAM PROCESSING (Syllabus) 2013-regulation Anna University

PY6012

DOWNSTREAM PROCESSING

 LPTC

3003

OBJECTIVE:
To introduce the student to the downstream processing and purification of biological products of relevance to the pharmaceutical, technical and food industries.

UNIT I

BIO PRODUCTS AND BIOSEPARATION-OVERVIEW

9

Broad classification of bioproducts - Small biomolecules - macromolecules, Stages of downstream processing. Cell disruption for product release – mechanical, enzymatic and chemical methods. Pretreatment and stabilisation of bioproducts.

UNIT II

PHYSICAL METHODS OF SEPARATION

9

Unit operations for solid-liquid separation – filtration - Conventional and cross flow filtration, Filter media and equipment. Sedimentation – Principle, methods, production centrifuges, ultracentrifugation, flocculation, membrane separation –ultrafiltration and reverse osmosis, dialysis,


UNIT III

ISOLATION OF PRODUCTS

9

Extraction- liquid-liquid extraction, solvent extraction principles, extraction process, aqueous two-phase extraction and its applications. Adsorption- Equilibrium and isotherms-adsorption column dynamics-fixed bed adsorption and agitated –bed adsorption.

UNIT IV

PRODUCT PURIFICATION

9

Classification of Chromatographic techniques – chromatographic terms and parameters principles, instruments and practice, adsorption, reverse phase, ion-exchange, size exclusion, hydrophobic interaction, bioaffinity and pseudo affinity chromatographic techniques.

UNIT V

FINAL PRODUCT FORMULATION AND FINISHING OPERATIONS

9

Precipitation of proteins, methods selective denaturation of unwanted proteins, Crystallization principles, batch crystallizers, crystallization of proteins, drying and lyophilization in final product formulation.

TOTAL : 45 PERIODS

OUTCOMES: The students will be able to
• Acquire theoretical knowlegde of unit operations: Filtration, Centrifugation, Protein precipitaition, Crystallisation, Drying, distillation, absorption, liquid-liquid extraction taking into account economic, and technical concerns.
• Calculate dimensions and choosing the internal structure of separation equipment for simple separation applications.
• gain knowledge of the structure and function of biological macromolecules and its separation

TEXT BOOKS:
1. Belter, P.A., Cussler, E.L. and Wei-Houhu., Bioseparations, Downstream Processing For Biotechnology, Wiley Interscience Publication, Singapore, 1st Edition, 1988
2. Asenjo, Juan A. “Separation Processes in Biotechnology”. Taylor & Francis CRC, 5th Edition,1990.

REFERENCES:
1. Ghosh, Raja “Principles of Bioseparations Engineering”. World Scientific, 2nd Edition 2006.
2. Jenkins, R.O., Product Recovery in Bioprocess Technology, Biotechnology by Open Learning Series, Butterworth- Heinemann, London, 2nd Edition, 1992
3. Harrison R.G., Todd P.W., Rudge S.R and Petrides D., Bioseparations science and engineering, Oxford University press, 2003

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