PY6012 - DOWNSTREAM PROCESSING (Syllabus) 2013-regulation Anna University
PY6012 - DOWNSTREAM PROCESSING (Syllabus) 2013-regulation Anna University
PY6012 |
DOWNSTREAM PROCESSING |
LPTC |
---|
3003
OBJECTIVE:
To introduce the student to the downstream processing and purification of biological products of relevance to the pharmaceutical, technical and food industries.
UNIT I |
BIO PRODUCTS AND BIOSEPARATION-OVERVIEW |
9 |
---|
Broad classification of bioproducts - Small biomolecules - macromolecules, Stages of downstream processing. Cell disruption for product release – mechanical, enzymatic and chemical methods. Pretreatment and stabilisation of bioproducts.
UNIT II |
PHYSICAL METHODS OF SEPARATION |
9 |
---|
Unit operations for solid-liquid separation – filtration - Conventional and cross flow filtration, Filter media and equipment. Sedimentation – Principle, methods, production centrifuges, ultracentrifugation, flocculation, membrane separation –ultrafiltration and reverse osmosis, dialysis,
UNIT III |
ISOLATION OF PRODUCTS |
9 |
---|
Extraction- liquid-liquid extraction, solvent extraction principles, extraction process, aqueous two-phase extraction and its applications. Adsorption- Equilibrium and isotherms-adsorption column dynamics-fixed bed adsorption and agitated –bed adsorption.
UNIT IV |
PRODUCT PURIFICATION |
9 |
---|
Classification of Chromatographic techniques – chromatographic terms and parameters principles, instruments and practice, adsorption, reverse phase, ion-exchange, size exclusion, hydrophobic interaction, bioaffinity and pseudo affinity chromatographic techniques.
UNIT V |
FINAL PRODUCT FORMULATION AND FINISHING OPERATIONS |
9 |
---|
Precipitation of proteins, methods selective denaturation of unwanted proteins, Crystallization principles, batch crystallizers, crystallization of proteins, drying and lyophilization in final product formulation.
TOTAL : 45 PERIODS
OUTCOMES: The students will be able to
• Acquire theoretical knowlegde of unit operations: Filtration, Centrifugation, Protein precipitaition, Crystallisation, Drying, distillation, absorption, liquid-liquid extraction taking into account economic, and technical concerns.
• Calculate dimensions and choosing the internal structure of separation equipment for simple separation applications.
• gain knowledge of the structure and function of biological macromolecules and its separation
• Calculate dimensions and choosing the internal structure of separation equipment for simple separation applications.
• gain knowledge of the structure and function of biological macromolecules and its separation
TEXT BOOKS:
1. Belter, P.A., Cussler, E.L. and Wei-Houhu., Bioseparations, Downstream Processing For Biotechnology, Wiley Interscience Publication, Singapore, 1st Edition, 1988
2. Asenjo, Juan A. “Separation Processes in Biotechnology”. Taylor & Francis CRC, 5th Edition,1990.
2. Asenjo, Juan A. “Separation Processes in Biotechnology”. Taylor & Francis CRC, 5th Edition,1990.
REFERENCES:
1. Ghosh, Raja “Principles of Bioseparations Engineering”. World Scientific, 2nd Edition 2006.
2. Jenkins, R.O., Product Recovery in Bioprocess Technology, Biotechnology by Open Learning Series, Butterworth- Heinemann, London, 2nd Edition, 1992
3. Harrison R.G., Todd P.W., Rudge S.R and Petrides D., Bioseparations science and engineering, Oxford University press, 2003
2. Jenkins, R.O., Product Recovery in Bioprocess Technology, Biotechnology by Open Learning Series, Butterworth- Heinemann, London, 2nd Edition, 1992
3. Harrison R.G., Todd P.W., Rudge S.R and Petrides D., Bioseparations science and engineering, Oxford University press, 2003
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