PY6403 - CELL AND MOLECULAR BIOLOGY (Syllabus) 2013-regulation Anna University

PY6403 - CELL AND MOLECULAR BIOLOGY (Syllabus) 2013-regulation Anna University

PY6403	CELL AND MOLECULAR BIOLOGY	LPTC

3003

OBJECTIVES:

• To provide the molecular processes of DNA replication, transcription and translation in cells.
• To provide the basic concepts of protein and nucleic acid structure and its function in both prokaryotic and eukaryotic organisms.

UNIT I	CELL STRUCTURE AND FUNCTION OF THE ORGANELLES	9

Prokaryotic, Eukaryotic cells, Sub-cellular organelles and functions. Purification of cell organelles, Principles of membrane organization of proteins, cytoskeleton proteins. Extra cellular matrix, cell-cell junctions, various types of transport across cell membrane.

UNIT II	CELL DIVISION, CANCER, APOPTOSIS AND IMMORTALIZATION OF CELLS	9

Cell cycle – Mitosis, Meiosis, Molecules controlling cell cycle, cancer, role of Ras and Raf in oncogenesis and apoptosis. Stem cells, immortalization of cells and its applications.

UNIT III	CHEMISTRY OF NUCLEIC ACIDS & DNA REPLICATION	12

Introduction to nucleic acids: Nucleic acids as genetic material, Structure and physicochemical properties of elements in DNA and RNA, Biological significance of differences in DNA and RNA. Structure of DNA: Primary, Secondary, Tertiary, DNA supercoiling; Overview of Central dogma; Organization of prokaryotic and eukaryotic chromosomes; DNA replication: Proteomics, Fidelity, Inhibitors and Overview of differences in prokaryotic and eukaryotic DNA replication; Telomere replication in eukaryotes.

UNIT IV	TRANSCRIPTION & TRANSLATION	9

Structure and function of mRNA, rRNA and tRNA. Characteristics of promoter and enhancer sequences. RNA synthesis: Proteomics, Fidelity, Inhibitors of transcription, Differences in prokaryotic and eukaryotic transcription. Basic concepts in RNA world: Ribozymes, RNA processing: 5’- Capping, Splicing-Alternative splicing, Poly ‘A’ tail addition and base modification. Introduction to Genetic code: Elucidation of genetic code, Codon degeneracy, Wobble hypothesis and its importance, Prokaryotic and eukaryotic ribosomes. Steps in translation: Initiation, Elongation and termination of protein synthesis. Inhibitors of protein synthesis. Post- translational modifications and its importance.

UNIT V	MUTAGENESIS, MUTATION AND MUTANTS	6

Biochemical basis of mutants, Mutagenesis-Base analogue mutagens, Chemical mutagens, Mutagenesis by intercalating substances, Site specific mutagenesis,Restriction Polymorphism to detect mutant sites in humans

TOTAL : 45 PERIODS

OUTCOMES: The student will be able to

• Understand the various levels of gene regulation and protein function including signal transduction and cell cycle control.
• Interpret the outcome of experiments that involve the use of recombinant DNA technology and other common gene analysis techniques.
• Relate properties of cancerous cells to mutational changes in gene function.

TEXT BOOKS:

1. Friefelder, David, “Molecular Biology”, 2nd Edition, Narosa Publishing House, 1999.
2. Lewin Benjamin, “Genes IX” Jones and Bartlett, 2008.
3. Weaver, R.F. “Molecular Biology”, 3rd Edition, McGraw Hill, 2005.

REFERENCES:

1. Waston, J.D. “Molecular Biology of the Gene”, 5th Edition, Pearson Education, 2004.
2. Karp, Gerald. “Cell and Molecular Biology: Concepts and Experiments.”2nd Edition, John Wiley & Sons, 1999.
3. Lodish, Harvey etal., “ Molecular Cell Biology,” 6th Edition. W.H.Freeman, 2008.