PY6501 - BIOCHEMICAL ENGINEERING (Syllabus) 2013-regulation Anna University

PY6501 - BIOCHEMICAL ENGINEERING (Syllabus) 2013-regulation Anna University

PY6501	BIOCHEMICAL ENGINEERING	LPTC

3003

OBJECTIVES:

• To provide the knowledge on fundamentals of Enzyme kinetics, Inhibition kinetics and Immobilization.
• To provide the concept of basic fermentation processes and its control systems during scale up operations.

UNIT I	INTRODUCTION TO ENZYMES	9

Classification of enzymes. Mechanisms of enzyme action; Principles of catalysis – collision theory, transition state theory; role of entropy in catalysis. Enzyme Kinetics- Single substrate reactions; Estimation of Michaelis – Menten parameters, Multi substrate reactions - Enzyme Immobilization and kinetics.

UNIT II	STERILIZATION KINETICS	9

Thermal death kinetics of microorganisms, batch and continuous heat sterilization of liquid media, filter sterilization of liquid media, air sterilization and design of sterilization equipment - batch and continuous.

UNIT III	METABOLIC STOICHIOMETRY AND ENERGETICS	9

Stoichiometry of cell growth and product formation, elemental balances, degrees of reduction of substrate and biomass, available electron balances, yield coefficients of biomass and product formation, maintenance coefficients, energetic analysis of microbial growth and product formation, oxygen consumption and heat evolution in aerobic cultures, thermodynamic efficiency of growth.

UNIT IV	KINETICS OF MICROBIAL GROWTH AND PRODUCT FORMATION UNSTRUCTURED KINETIC MODELS	9

Modes of operation - batch, fed batch and continuous cultivation. Simple unstructured kinetic models for microbial growth, Monod model, growth of filamentous organisms. Types of reactor- Air Lift Reactor, Bubble Column Reactor, Immobilized enzyme reactors- packed bed, fluidized bed, membrane reactors.

UNIT V	BIOREACTOR SCALE – UP	9

Regime analysis of bioreactor processes, oxygen mass transfer in bioreactors – Mass transfer Coefficient- methods for the determination of mass transfer coefficients; mass transfer correlations. Power requirements of Bioreactors. Scale-up considerations on heat transfer oxygen transfer, power consumption and impeller tip speed.

TOTAL : 45 PERIODS

OUTCOMES: The student will be able to

• Understand the fundamentals of Enzyme kinetics, Inhibition kinetics and Immobilization
• Understand the concept of basic fermentation processes and its application during scale up operations.

TEXT BOOKS:

1. Shuler, M.L. and Kargi, F. “Bioprocess Engineering: Basic Concepts”, 2nd Edition, PHI, 2002.
2. Bailey, J.E. and Ollis, D.F. “Biochemical Engineering Fundamentals” 2nd Edition, McGraw – Hill, 1988.

REFERENCES:

1. Wiseman, Alan “Handbook of Enzyme Biotechnology”, 3rd Edition, Ellis Harwood Publications, 1999.
2. Moser, Anton. “Bioprocess Technology : Kinetics and Reactors”, Springer – Verlag, 1st Edition, 1988.
3. Stanbury, P.F. et al. “Principles of Fermentation Technology”, Butterworth – Heinemann/Elsevier, 2nd Edition, 1995.