PY8008 - VACCINE TECHNOLOGY (Syllabus) 2017-regulation Anna University

PY8008 - VACCINE TECHNOLOGY (Syllabus) 2017-regulation Anna University

PY8008	VACCINE TECHNOLOGY	LPTC

3003

OBJECTIVES:

• To provide the knowledge on conventional to recent technology of vaccine production.
• To learn the types of vaccines, its immunological effects and regulatory guidelines.

UNIT I	IMMUNOLOGICAL CONCEPTS IN VACCINOLOGY	9

Short history of vaccination, requirements for induction of immunity, Epitopes, linear and conformational epitopes, characterisation and location of APC, MHC and immunogenicity, Rationale vaccine design based on clinical requirements: Hypersensitivity, Immunity to Infection, Autoimmunity, immunodeficiency, mechanism of adjuvant action, Scope of future vaccine strategies.

UNIT II	CLASSIFICATION OF VACCINES AND ITS PREPARATIONS	10

Active and passive immunization; Viral/bacterial/parasite vaccine differences, methods of vaccine preparation – Live, killed, attenuated, sub unit vaccines; Vaccine technology- Role and properties of adjuvants, recombinant DNA and protein based vaccines, plant-based vaccines, edible vaccines, reverse vaccinology, combination vaccines, therapeutic vaccines; Peptide vaccines, conjugate vaccines; Cell based vaccines.

UNIT III	VACCINE RESEARCH AND DESIGN	9

Fundamental research to rational vaccine design. Antigen identification and delivery, T-Cell expression cloning for identification of vaccine targets for intracellular pathogens, Fundamentals of Immune recognition, implications for manipulating the T-Cell repertoire, Targeting Dendritic cells; a rational approach for Vaccine development, Cellular basis of T- Cell memory, Rational design of new vectors, CpG adjuvant activity, Transcutaneous immunisation, Vaccination studies and recent advances in Malaria, Tuberculosis, HIV.

UNIT IV	COMPUTATIONAL TOOLS FOR VACCINE DESIGN	8

Antigen Sequence analysis, Epitope Mapping, Predictions of Immunogenic peptides of T-Cell and B-Cells. Prediction of HLA binding peptides, Comparative Genomics as a tool for vaccine design, introduction to online epitope databases.

UNIT V	ANIMAL TESTING, COMMERCIALISATION, QUALITY CONTROL	9

Quality control and regulations in vaccine research, In-vitro experimental validations for predictions of vaccines by software, Animal testing, Rational design to clinical trials, Large scale production, Commercialisation, ethics.

TOTAL : 45 PERIODS

OUTCOMES: The students after completing the course

• Aware of the strategies available for developing an innovative vaccine technology with different mode of vaccine delivery.
• Able to explain the significance of critical antigens, immunogens and adjuvants in developing effective vaccines.
• Aware of the regulatory issues, guidelines for the management of production of vaccine.

TEXT BOOKS:

1. Ronald W. Ellis, “New Vaccine Technologies”, Landes Bioscience, 2001.
2. Cheryl Barton, “Advances in Vaccine Technology and Delivery”, Espicom Business Intelligence, 2009.
3. Male, David et al., “Immunology”, 7th Edition, Mosby Publication, 2007.

REFERENCES:

1. Coico, R. etal., “Immunology: A Short Course”, 5th Edition, Wiley – Liss, 2003.
2. Parham, Peter “The Immune System”, 2nd Edition, Garland Science, 2005.
3. Abbas, A.K. etal., “The Cellular and Molecular Immunology”, 6th Edition, Sanders / Elsevier, 2007.
4. Weir, D.M. and Stewart, John “Immunology”, 8th Edition, Churchill Pvt. Ltd., 2000