PY8202 - BIOCHEMISTRY (Syllabus) 2017-regulation Anna University

PY8202 - BIOCHEMISTRY (Syllabus) 2017-regulation Anna University

PY8202	BIOCHEMISTRY	LPTC

3003

OBJECTIVE:

• To enable students learn the fundamentals of Biochemical Processes and Biomolecules.

UNIT I	BIOCHEMICAL ORGANIZATION AND BIOENERGETICS	8

Scope of clinical biochemistry, component of the cell, structure and biochemical functions, membrane structure and functions, transport through biological cell membrane, transport mechanism, the concept of free energy.

UNIT II	BIOMOLECULES	12

Carbohydrates – classification, properties. starch, glycogen, dextrin, inulin, cellulose, metabolism of carbohydrates – gluconeogenesis, glycogenolysis, glycolysis, citric acid cycle and its biological significance, role of sugar in nucleotide biosynthesis and pentose phosphate pathway. Lipids – Classification, properties- sterols, essential fatty acids, eicosanoids, phospholipids, sphingolipids, oxidation of fatty acids, α,β - oxidation and biosynthesis of cholesterol and porphyrin Proteins and amino acids – Classification, properties, essential and non essential amino acids, biosynthesis of amino acids. Nucleic acids –genetic code, nucleic acids, and structure of DNA and RNA, purine biosynthesis and pyrimidine biosynthesis.

UNIT III	BIOENERGETICS	7

High energy compounds, respiratory chain, ATP cycle, Calculation of ATP during oxidation of glucose and fatty acids. General concept of oxidation and reduction, electron transport chain, oxidative phosphorylation, uncouplers and theories of biological oxidation, chemiosmotic hypothesis.

UNIT IV	MACROMOLECULES, VITAMINS, HORMONES, ENZYMES	10

Structure of haemoglobin, immunoglobulins and nucleoprotein, classification and their properties, occurrence, functions, requirements, deficiency manifestations and role of vitamins as coenzyme, chemical nature and properties, hormones, Enzyme classification and their properties, mechanism of action, enzyme induction and inhibition, coenzyme significance and enzymes of clinical importance.

UNIT V	BIOCHEMISTRY OF CLINICAL DISEASES	8

Diabetes mellitus, atherosclerosis, Renal failure and obesity, hormonal disorders, aging, inborn errors of metabolism organ function tests

TOTAL : 45 PERIODS

TEXT BOOKS:

1. Lehninger A.L., Nelson D.L. and Cox M.M. Principles of Biochemistry. CBS publishers and distributers
2. Murray R.K., Granner D.K., Mayes P.A. and Rodwell V.W. Harpers Biochemistry. Appleton and Lange , Stanford ,Conneticut.
3. Thomas M. Devlin.Textbook of Biochemistry with clinical correlations. Wiley Liss Publishers

REFERENCES:

1. Burtis & Ashwood W.B. Tietz Textbook of Clinical chemistry. Saunders Company
2. Lubert Stryer W.H. Biochemistry. Freeman and company, New york.
3. Donald Voet & Judith G. Voet. Biochemistry. John Wiley and Sons ,Inc.
4. Rama Rao. Textbook of Biochemistry.
5. Deb. Textbook of Biochemistry.