PY8404 - MOLECULAR BIOLOGY AND GENETIC ENGINEERING (Syllabus) 2017-regulation Anna University

PY8404 - MOLECULAR BIOLOGY AND GENETIC ENGINEERING (Syllabus) 2017-regulation Anna University

PY8404

MOLECULAR BIOLOGY AND GENETIC ENGINEERING

 LPTC

3003

OBJECTIVES:
• To expose students to application of recombinant DNA technology in biotechnological research.
• To train students in strategizing research methodologies employing cloning, construction of DNA libraries
• To illustrate creative use of modern tools and techniques for manipulation and analysis of genomic sequences.

UNIT I

MOLECULAR GENETICS

12

Bacterial conjugation, transduction and transformation, prokaryotic and eukaryotic genome organization; Introduction to nucleic acids, Nucleic acids as genetic material, Structure and function of DNA and RNA, DNA replication, Overview of differences in prokaryotic and eukaryotic DNA replication, Telomere replication in eukaryotes. Mutagens, DNA mutations and their mechanism, various types of repair mechanisms.

UNIT II

TRANSCRIPTION AND TRANSLATION

12

Structure and function of mRNA, rRNA and tRNA. RNA synthesis: Initiation, elongation and termination of RNA synthesis, Translation: Introduction to Genetic code: Elucidation of genetic code, Codon degeneracy, Wobble hypothesis and its importance, Steps in translation: Initiation, Elongation and termination of protein synthesis. Inhibitors of protein synthesis. Post-translational modifications and its importance. Organization of genes in prokaryotic and eukaryotic chromosomes.


UNIT III

RECOMBINANT DNA TECHNOLOGY

12

Manipulation of DNA – Restriction and Modification enzymes. Characteristics of cloning and expression vectors based on plasmid and bacteriophage, Vectors for yeast, insect and mammalian systems, Prokaryotic and eukaryotic expression host systems, Introduction of recombinant DNA in to host: Insulin, Interferons, Erythropoietin, DNA libraries: Construction of genomic and cDNA libraries.

UNIT IV

SEQUENCING AND AMPLIFICATION OF DNA

12

Amplification of DNA; Types of PCR, Real-time PCR/qPCR – SYBR green assay, Taqman assay, Site directed mutagenesis. Organization and structure of genomes, Maxam Gilbert’s and Sanger Coulson’s and automated methods of DNA sequencing, Next generation sequencing technologies, Genetic maps and Physical maps.

UNIT V

GENOME ANALYSIS AND GENOMICS

12

Gene therapy and Transgenic technology, Introduction to Functional genomics, Microarrays, Serial Analysis of Gene expression (SAGE), Web resources for Genomics, Regulation of Eukaryotic Gene Expression by Small RNAs (RNA Interference, RNAi).

TOTAL : 60 PERIODS

OUTCOMES: By the end of this course, students will be able to
• Describe the basic structure of nucleic acids, identify the principles of DNA replication, transcription and translation of proteins
• To produce the commercially important recombinant proteins
• Understand about gene expression and genome sequencing techniques

TEXT BOOKS:
1. David Friedfeld “Molecular Biology.” Narosa Publications, 1999.
2. Primrose SB and R. Twyman “Principles of Gene Manipulation & Genomic Blackwell Science Publications, 2006.
3. Principles of Genome Analysis and Genomics by S.B. Primrose and R.M. Twyman, Third Edition (Blackwell Publishing), 2003.

REFERENCES:
1. Tropp, Burton. “Molecular Biology: Genes to Proteins”. 3rd Edition. Jones andBartlett, 2008.
2. Ansubel FM, Brent R, Kingston RE, Moore DD, “Current Protocols in Molecular Biology“Greene Publishing Associates, NY, 1998
3. Genomes 3 by T.A.Brown, Third Edition (Garland Science Publishing), 2007.

Comments

Popular posts from this blog

CS3491 Syllabus - Artificial Intelligence And Machine Learning - 2021 Regulation Anna University

CS3401 Syllabus - Algorithms - 2021 Regulation Anna University

MA3251 - STATISTICS AND NUMERICAL METHODS (Syllabus) 2021-regulation Anna University