BM3271 - Biosciences Laboratory (Syllabus) 2021-regulation Anna University
BM3271 - Biosciences Laboratory (Syllabus) 2021-regulation Anna University
BM3271 |
BIOSCIENCES LABORATORY |
LTPC |
|---|
0042
COURSE OBJECTIVES: To provide practice on:
• Estimation and quantification of biomolecules.
• Separation of macromolecules.
• Use Compound microscope
• Practice on chemical examinations, Histopathological examinations etc
• Separation of macromolecules.
• Use Compound microscope
• Practice on chemical examinations, Histopathological examinations etc
LIST OF EXPERIMENTS:
1. Preparation of solutions: 1) percentage solutions, 2) molar solutions, 3) normal solutions
2. Standardization of pH meter, preparation of buffers, emulsions.
3. Spectroscopy: Determination of absorption maxima (λmax) of a given solution
4. General tests for carbohydrates, proteins and lipids.
5. Identification of Blood Collection Tubes and Phlebotomy equipment
6. Preparation of serum and plasma from blood
7. Estimation of Haemoglobin and blood glucose
8. Estimation of creatinine, urea and Uric acid
9. Separation of proteins by SDS electrophoresis(Demo) and amino acids by thin layer chromatography (Demo).
10. Urine physical and chemical examination (protein, reducing substances, ketones, bilirubin and blood)
11. Basic staining – Hematoxylin and eosin staining.
12. Special stains – cresyl fast Blue (CFV)- Trichrome – oil red O – PAS
13. Types of Staining : Simple stain, Gram stain
14. Study of parts of compound microscope
15. Study of Histopathological slides of benign and malignant tumours.
16. Study of Haematology slides of anemia and leukemia.
2. Standardization of pH meter, preparation of buffers, emulsions.
3. Spectroscopy: Determination of absorption maxima (λmax) of a given solution
4. General tests for carbohydrates, proteins and lipids.
5. Identification of Blood Collection Tubes and Phlebotomy equipment
6. Preparation of serum and plasma from blood
7. Estimation of Haemoglobin and blood glucose
8. Estimation of creatinine, urea and Uric acid
9. Separation of proteins by SDS electrophoresis(Demo) and amino acids by thin layer chromatography (Demo).
10. Urine physical and chemical examination (protein, reducing substances, ketones, bilirubin and blood)
11. Basic staining – Hematoxylin and eosin staining.
12. Special stains – cresyl fast Blue (CFV)- Trichrome – oil red O – PAS
13. Types of Staining : Simple stain, Gram stain
14. Study of parts of compound microscope
15. Study of Histopathological slides of benign and malignant tumours.
16. Study of Haematology slides of anemia and leukemia.
TOTAL : 60 PERIODS
COURSE OUTCOMES: Upon completion of the course, students will be able to:
• Understand the Biochemistry laboratory functional components
• Have a sound knowledge of qualitative test of different biomolecules.
• Understand the basics knowledge of Biochemical parameter and their interpretation in Blood sample.
• Have a sound knowledge of separation technology of proteins and amino acids.
• Student can perform practical experiments on staining Processes.
• Have a sound knowledge of qualitative test of different biomolecules.
• Understand the basics knowledge of Biochemical parameter and their interpretation in Blood sample.
• Have a sound knowledge of separation technology of proteins and amino acids.
• Student can perform practical experiments on staining Processes.
TEXT BOOK:
1. Ramnik Sood, Textbook of Medical Laboratory Technology, 6thEdition, Jaypee Brothers Medical Publishers, 2009
LAB REQUIREMENT FOR A BATCH OF 30 STUDENTS:
Requirement for a batch of 30 students
Colorimeter 2 Nos
Spectrophotometer 1 No.
pH meter 1 No
Weighing balance 1 No
Refrigerator 1 No
SDS gel electrophoresis 1 No
TLC, ready TLC plates 1 No
Wintrobe’s tube 2 Nos.
Centrifuge Normal 1 No
Microslides 2 packets
Lancet 5 boxes
Microscope 1 No
Neubaur’s Chamber 2 Nos.
Heparinized Syringe 1box
Haemoglobinometer 1 No
Capillary tubes 1 box
Colorimeter 2 Nos
Spectrophotometer 1 No.
pH meter 1 No
Weighing balance 1 No
Refrigerator 1 No
SDS gel electrophoresis 1 No
TLC, ready TLC plates 1 No
Wintrobe’s tube 2 Nos.
Centrifuge Normal 1 No
Microslides 2 packets
Lancet 5 boxes
Microscope 1 No
Neubaur’s Chamber 2 Nos.
Heparinized Syringe 1box
Haemoglobinometer 1 No
Capillary tubes 1 box
Comments
Post a Comment