BT3351 Syllabus - Cell Biology - 2021 Regulation Anna University

BT3351 Syllabus - Cell Biology - 2021 Regulation Anna University

BT3351 CELL BIOLOGY  LTPC
3003

COURSE OBJECTIVES:
• To provide knowledge on the fundamentals of cell biology.
• To help students understand the signalling mechanisms.

UNIT I CELL STRUCTURE AND FUNCTION OF THE ORGANELLES 9
Prokaryotic, Eukaryotic cells, Sub-cellular organelles and functions. Principles of membrane organization membrane proteins, cytoskeletal proteins. Extra cellular matrix, cell-cell junctions.

UNIT II CELL DIVISION, CANCER, APOPTOSIS AND IMMORTALIZATION OF CELLS 9
Cell cycle – Mitosis, Meiosis, Molecules controlling cell cycle, cancer, role of Ras and Raf in oncogenesis and apoptosis. Stem cells, Cell culture and immortalization of cells and its applications.


UNIT III TRANSPORT ACROSS CELL MEMBRANE 9
Passive and Active Transport, Permeases, Ion channels, ATP pumps. Na+ / K+ /Ca+2Tpumps, uniport, symport antiporter system. Ligand gated / voltage gated channels, Agonists and Antagonists.

UNIT IV SIGNAL TRANSDUCTION 9
Receptors – extracellular signaling, Cell surface / cytosolic receptors and examples, Differentclasses of receptors antocrine / paracrine / endocrine models, Secondary messengers molecules

UNIT V TECHNIQUES USED TO STUDY CELLS 9
Cell fractionation and flow cytometry, Morphology and identification of cells using microscopic studies like SEM, TEM and Confocal Microscopy. Localization of proteins in cells – Immunostaining.

TOTAL: 45 PERIODS

COURSE OUTCOMES: Upon completion of this course, the students
1. Would have deeper understanding of cell at structural and functional level.
2. Would have broad knowledge on the molecular interaction between cells.
3. Would demonstrate a clear understanding of the signal transduction, secondary messengers.
4. Would develop skill on working principles of microscopy and identification of cell types.

TEXT BOOKS:
1. Lodish, Harvey etal., “Molecular Cell Biology”, 7th Edition, W.H.Freeman, 2005.
2. Cooper, G.M. and R.E. Hansman “The Cell: A Molecular Approach”, VIIth Edition, ASM Press, 2007.
3. Alberts, Bruce etal., “Molecular Biology of the Cell”, IVth Edition, Garland Science (Taylors Francis), 2002.
4. Sadava, D.E. “Cell Biology: Organelle Structure and Function”, Panima Publishing, 2004.
5. Rastogi, S.C. “Cell Biology” IInd Edition, New Age International, 2002.

REFERENCES:
1. Becker, W.M. etal., “The World of the Cell”, 9th Edition, Pearson Education, 2003.
2. Campbell, N.A., J.B. Recee and E.J. Simon “Essential Biology”, VIIrd Edition, Pearson International, 2007.
3. Alberts, Bruce etal., “Essential Cell Biology”, IVth Edition, Garland Press (Taylor & Francis), 2004.
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