BT3392 Syllabus - Biochemistry - 2021 Regulation Anna University

BT3392 Syllabus - Biochemistry - 2021 Regulation Anna University

BT3392	BIOCHEMISTRY	LTPC

3003

COURSE OBJECTIVES:

• To enable students learn the fundamentals of Biochemical Processes and Biomolecules

UNIT I	INTRODUCTION TO BIOMOLECULES - CARBOHYDRATES:	9

Basic principles of organic chemistry, role of carbon, types of functional groups, chemical, nature of water, pH and biological buffers, bio molecules structure and properties of Carbohydrates (mono, di, oligo & polysaccharides) Proteoglycans, glucosaminoglycans. mutarotation, glycosidic 58 bond, reactions of monosaccharides, reducing sugars. Starch, glycogen, cellulose and chitin. Proteoglycans, glycosaminoglycans. hyaluronic acid, chondroitin sulfate

UNIT II	STRUCTURE AND PROPERTIES OF OTHER BIOMOLECULES	9

Structure and properties of Important Biomolecules. Lipids: fatty acids, glycerol, saponification, iodination, hydrogenation, phospholipids, glycolipids, sphingolipids, cholesterol, steroids, prostaglandins. Protein: Amino Acids, Peptides, Proteins, measurement, structures, hierarchy of organization primary, secondary, tertiary and quaternary structures, glycoproteins, lipoproteins. Determine of primary structure. Nucleic acids: purines, pyrimidines, nucleoside, nucleotide, RNA, DNA-Watson-Crick structure of DNA, reactions, properties, measurement, nucleoprotein complexes

UNIT III	METABOLISM CONCEPTS AND CARBOHYDRATE METABOLISM	9

Functions of Proteins, Enzymes, introduction to biocatalysts, metabolic pathways, primary and secondary metabolites. Interconnection of pathways and metabolic regulation. Glycolysis, TCA cycle, gluconeogenesis, pentose phosphate shunt & glyoxalate shunt.

UNIT IV	INTERMEDIARY METABOLISM AND REGULATION	9

Fatty acid synthesis and oxidation, reactions of amino acids, deamination, transamination and decarboxylation, urea cycle, Bioenergetics - High energy compounds, electronegative potential of compounds, respiratory chain, ATP cycle, calculation of ATP yield during oxidation of glucose and fatty acids.

UNIT V	PROTEIN TRANSPORT AND DEGRADATION	9

Protein targeting, signal sequence, secretion; Folding, Chaperone and targeting of organelle proteins, Protein degradation, receptor-mediated endocytosis, turnover.

TOTAL: 45 PERIODS

COURSE OUTCOMES:

1. To ensure students have a strong foundation in the structure and reactions of Biomolecules.
2. To introduce them to metabolic pathways of the major biomolecules and relevance to clinical conditions.
3. To correlate Biochemical processes with Biotechnology applications.

TEXT BOOKS:

1. Lehninger Principles of Biochemistry 6th Edition by David L. Nelson, Michael M. Cox
2. Satyanarayana, U. and U. Chakerapani, “Biochemistry” 3rd Rev. Edition, Books & Allied (P) Ltd., 2006.
3. Rastogi, S.C. “Biochemistry” 2nd Edition, Tata McGraw-Hill, 2003.
4. Conn, E.E., etal., “Outlines of Biochemistry” 5th Edition, John Wiley & Sons, 1987.
5. Outlines of biochemistry, 5th Edition: By E E Conn, P K Stumpf, G Bruening and R Y Doi. pp 693. John Wiley and Sons, New York. 1987.

REFERENCES:

1. Berg, Jeremy M. et al. “Biochemsitry”, 6 th Edition, W.H. Freeman & Co., 2006.
2. Murray, R.K., etal “Harper’s Illustrated Biochemistry”, 27th Edition, McGraw-Hill, 2006. 3. Voet, D. and Voet, J.G., “Biochemistry”, 3rd Edition, John Wiley & Sons Inc.,2004.