BT3451 Syllabus - Analytical Techniques In Biotechnology - 2021 Regulation Anna University
BT3451 Syllabus - Analytical Techniques In Biotechnology - 2021 Regulation Anna University
BT3451 | ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY | LTPC |
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3003
COURSE OBJECTIVES:
To enable the students
• To have a fundamental knowledge about the Light spectrum, Absoprtion, Fluorescence, NMR, Mass spectroscopy.
• To acquire knowledge on the different chromotographic methods for separation of biological products.
• To have a fundamental knowledge about the Light spectrum, Absoprtion, Fluorescence, NMR, Mass spectroscopy.
• To acquire knowledge on the different chromotographic methods for separation of biological products.
UNIT I | INTRODUCTION TO SPECTROMETRY | 9 |
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Properties of electromagnetic radiation- wave properties – components of
optical instruments – Sources of radiation – wavelength selectors – sample
containers – radiation transducers – Signal process and read outs – signal
to noise ratio - sources of noise – Enhancement of signal to noise - types
of optical instruments – Principle of Fourier Transform optical
Measurements.
UNIT II | MOLECULAR SPECTROSCOPY | 9 |
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Molecular absorption spectrometry – Measurement of Transmittance and
Absorbance – Beer’s law – Instrumentation - Applications -Theory of
fluorescence and Phosphorescence – Instrumentation 71 – Applications –
Theory of Infrared absorption spectrometry – IR instrumentation –
Applications – Theory of Raman spectroscopy – Instrumentation –
applications
UNIT III | MAGNETIC RESONANCE SPECTROSCOPY AND MASS SPECTROMETRY | 9 |
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Theory of NMR – environmental effects on NMR spectra – chemical shift-
NMR-spectrometers – applications of 1H and 13C NMR- Molecular mass spectra
– ion sources – Mass spectrometer. Applications of molecular mass -
Electron paramagnetic resonance- g values –instrumentation.
UNIT IV | SEPARATION METHODS | 9 |
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General description of chromatography – Band broadening and optimization
of column performance- Liquid chromatography – Partition chromatography –
Adsorption chromatography – Ion exchange chromatography -size exclusion
chromatography- Affinity chromatographyprinciples of GC and applications –
HPLC- Capillary electrophoresis – Applications.
UNIT V | ELECTRO ANALYSIS AND SURFACE MICROSCOPY | 9 |
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Electrochemical cells- Electrode potential cell potentials –
potentiometry- reference electrode – ion selective and molecular selective
electrodes – Instrument for potentiometric studies – Voltametry – Cyclic
and pulse voltametry- Applications of voltametry . Study of surfaces –
Scanning probe microscopes – AFM and STM.
TOTAL: 45 PERIODS
COURSE OUTCOMES: On completion of the course, students will
1. have a better understanding of spectroscopy andthe separation
techniques used for biological products.
TEXT BOOKS:
1. Skoog, D.A. F. James Holler, and Stanky, R.Crouch “Instrumental Methods
of Analysis”.CengageLearning , 2007.
2. Willard, Hobart, etal., “Instrumental Methods of Analysis”. VIIth Edition, CBS, 1986.
3. Fifield F.W., . Principles and Practice of Analytical Chemistry. Blackwell, Scientific Publishers,2016
2. Willard, Hobart, etal., “Instrumental Methods of Analysis”. VIIth Edition, CBS, 1986.
3. Fifield F.W., . Principles and Practice of Analytical Chemistry. Blackwell, Scientific Publishers,2016
REFERENCES:
1. Sharma, B.K. “Instrumental Methods of Chemical Analysis: Analytical
Chemistry”, Krishna Prakashan Media (P) Ltd, 2014
2. Haven, Mary C., etal., “Laboratory Instrumentation “. 4th Edition, Wiley India Pvt Ltd, 2010
3. Philopose P.M.Analytical Biotechnology. Domihant Publishers & distributors, New Delhi, 2016.
2. Haven, Mary C., etal., “Laboratory Instrumentation “. 4th Edition, Wiley India Pvt Ltd, 2010
3. Philopose P.M.Analytical Biotechnology. Domihant Publishers & distributors, New Delhi, 2016.
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