BT3451 Syllabus - Analytical Techniques In Biotechnology - 2021 Regulation Anna University

BT3451 Syllabus - Analytical Techniques In Biotechnology - 2021 Regulation Anna University

BT3451 ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY  LTPC
3003

COURSE OBJECTIVES:
To enable the students
• To have a fundamental knowledge about the Light spectrum, Absoprtion, Fluorescence, NMR, Mass spectroscopy.
• To acquire knowledge on the different chromotographic methods for separation of biological products.

UNIT I INTRODUCTION TO SPECTROMETRY 9
Properties of electromagnetic radiation- wave properties – components of optical instruments – Sources of radiation – wavelength selectors – sample containers – radiation transducers – Signal process and read outs – signal to noise ratio - sources of noise – Enhancement of signal to noise - types of optical instruments – Principle of Fourier Transform optical Measurements.

UNIT II MOLECULAR SPECTROSCOPY 9
Molecular absorption spectrometry – Measurement of Transmittance and Absorbance – Beer’s law – Instrumentation - Applications -Theory of fluorescence and Phosphorescence – Instrumentation 71 – Applications – Theory of Infrared absorption spectrometry – IR instrumentation – Applications – Theory of Raman spectroscopy – Instrumentation – applications


UNIT III MAGNETIC RESONANCE SPECTROSCOPY AND MASS SPECTROMETRY 9
Theory of NMR – environmental effects on NMR spectra – chemical shift- NMR-spectrometers – applications of 1H and 13C NMR- Molecular mass spectra – ion sources – Mass spectrometer. Applications of molecular mass - Electron paramagnetic resonance- g values –instrumentation.

UNIT IV SEPARATION METHODS 9
General description of chromatography – Band broadening and optimization of column performance- Liquid chromatography – Partition chromatography – Adsorption chromatography – Ion exchange chromatography -size exclusion chromatography- Affinity chromatographyprinciples of GC and applications – HPLC- Capillary electrophoresis – Applications.

UNIT V ELECTRO ANALYSIS AND SURFACE MICROSCOPY 9
Electrochemical cells- Electrode potential cell potentials – potentiometry- reference electrode – ion selective and molecular selective electrodes – Instrument for potentiometric studies – Voltametry – Cyclic and pulse voltametry- Applications of voltametry . Study of surfaces – Scanning probe microscopes – AFM and STM.

TOTAL: 45 PERIODS

COURSE OUTCOMES: On completion of the course, students will
1. have a better understanding of spectroscopy andthe separation techniques used for biological products.

TEXT BOOKS:
1. Skoog, D.A. F. James Holler, and Stanky, R.Crouch “Instrumental Methods of Analysis”.CengageLearning , 2007.
2. Willard, Hobart, etal., “Instrumental Methods of Analysis”. VIIth Edition, CBS, 1986.
3. Fifield F.W., . Principles and Practice of Analytical Chemistry. Blackwell, Scientific Publishers,2016

REFERENCES:
1. Sharma, B.K. “Instrumental Methods of Chemical Analysis: Analytical Chemistry”, Krishna Prakashan Media (P) Ltd, 2014
2. Haven, Mary C., etal., “Laboratory Instrumentation “. 4th Edition, Wiley India Pvt Ltd, 2010
3. Philopose P.M.Analytical Biotechnology. Domihant Publishers & distributors, New Delhi, 2016.
Previous
Next

Comments

Post a Comment

Popular posts from this blog

EC8094 - SATELLITE COMMUNICATION (Syllabus) 2017-regulation Anna University

EC8094 - SATELLITE COMMUNICATION (Syllabus) 2017-regulation Anna University EC8094 SATELLITE COMMUNICATION  LPTC 3003 OBJECTIVES: • Understand the basics of satellite orbits • Understand the satellite segment and earth segment • Analyze the various methods of satellite access • Understand the applications of satellites • Understand the basics of satellite Networks UNIT I SATELLITE ORBITS       9 Kepler‟s Laws, Newton‟s law, orbital parameters, orbital perturbations, station keeping, geo stationary and non Geo-stationary orbits – Look Angle Determination- Limits of visibility – eclipse-Sub satellite point –Sun transit outage-Launching Procedures - launch vehicles and propulsion. UNIT II SPACE SEGMENT       9 Spacecraft Technology- Structure, Primary power, Attitude and Orbit control, Thermal control and Propulsion, communication Payload and supporting subsystems, Telemetry, Tracking and
Read more

HS8581 - PROFESSIONAL COMMUNICATION (Syllabus) 2017-regulation Anna University

HS8581 - PROFESSIONAL COMMUNICATION (Syllabus) 2017-regulation Anna University HS8581 PROFESSIONAL COMMUNICATION  LPTC 3003 OBJECTIVES: The course aims to: • Enhance the Employability and Career Skills of students • Orient the students towards grooming as a professional • Make them Employability Graduates • Develop their confidence and help them attend interviews successfully. UNIT I Introduction to Soft Skills-- Hard skills & soft skills - employability and career Skills—Grooming as a professional with values—Time Management—General awareness of Current Affairs UNIT II Self-Introduction-organizing the material - Introducing oneself to the audience – introducing the topic – answering questions – individual presentation practice–– presenting the visuals effectively – 5 minute presentations UNIT III Introduction to Group
Read more

OML751 - TESTING OF MATERIALS (Syllabus) 2017-regulation - Open Elective | Anna University

OML751 - TESTING OF MATERIALS (Syllabus) 2017-regulation - Open Elective | Anna University OML751 TESTING OF MATERIALS  LPTC 3003 OBJECTIVE: To understand the various destructive and non destructive testing methods of materials and its industrial applications. UNIT I INTRODUCTION TO MATERIALS TESTING 9 Overview of materials, Classification of material testing, Purpose of testing, Selection of material, Development of testing, Testing organizations and its committee, Testing standards, Result Analysis, Advantages of testing. UNIT II MECHANICAL TESTING 9 Introduction to mechanical testing, Hardness test (Vickers, Brinell, Rockwell), Tensile test, Impact test (Izod, Charpy) - Principles, Techniques, Methods, Advantages and Limitations, Applications. Bend test, Shear test, Creep and Fatigue test - Principles, Techniques, Methods, Advantages and Limitations, Applications.
Read more