BT3452 Syllabus - Industrial Enzymology - 2021 Regulation Anna University

BT3452 Syllabus - Industrial Enzymology - 2021 Regulation Anna University

BT3452 INDUSTRIAL ENZYMOLOGY  LTPC
3003

COURSE OBJECTIVES:
To enable the students
• To learn enzyme reactions and its characteristics along with the production and purification process.
• To give the student a basic knowledge concerning biotransformation reactions with the usage of enzymes

UNIT I INTRODUCTION TO ENZYMES 9
Classification of enzymes. Mechanisms of enzyme action; concept of active site and energetics of enzyme substrate complex formation; specificity of enzyme action; principles of catalysis – collision theory, transition state theory; role of entropy in catalysis.

UNIT II KINETICS OF ENZYME ACTION 9
Kinetics of single substrate reactions; estimation of Michelis – Menten parameters, multisubstrate reactions - mechanisms and kinetics; turnover number; types of inhibition & models –substrate, product. Allosteric regulation of enzymes, Monod Changeux Wyman model, pH and temperature effect on enzymes & deactivation kinetics.


UNIT III ENZYME IMMOBILIZATION AND BIOSENSORS 9
Physical and chemical techniques for enzyme immobilization – adsorption, matrix entrapment, encapsulation, cross-linking, covalent binding etc., - examples, advantages and disadvantages, design of enzyme electrodes and their application as biosensors in industry, healthcare and environment.

UNIT IV PURIFICATION AND CHARACTERIZATION OF ENZYMES FROM NATURAL SOURCES 9
Production and purification of crude enzyme extracts from plant, animal and microbial sources; methods of characterization of enzymes; development of enzymatic assays

UNIT V BIOTRANSFORMATION APPLICATIONS OF ENZYMES 9
Hydrolytic- Ester bond, Amide, Epoxides, Nitriles, Reduction reactions –aldehydes, Ketones, C=C, Oxidation reactions – Alkanes, Aromatic, Baeyer-Villiger, Enzymes in organic synthesis – esters, amide, peptide , Modified and Artificial Enzymes , Catalytic antibodies

TOTAL: 45 PERIODS

COURSE OUTCOMES:
1. The knowledge on enzyme and enzyme reactions will be the key step in to proceed towards various concepts in biotechnology.
2. The theoretical and practical aspects of kinetics will provide the importance and utility of enzyme kinetics towards research.
3. The process of immobilization has been increased steadily in food, pharmaceutical and chemical industries and thus this study will provide simple and easy method of implementation.
4. Ideas on Processing, Production and Purification of enzymes at an industrial scale will be helpful to work technologically.

TEXT BOOKS:
1. Trevor Palmer , Enzymes IInd Horwood Publishing Ltd
2. Faber K , Biotransformations in Organic Chemistry, IV edition , Springer

REFERENCES:
1. Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering, Marcel Dekker, Inc. 74
2. James M. Lee, Biochemical Engineering, PHI, USA.
3. James. E. Bailey & David F. Ollis, Biochemical Engineering Fundamentals, McGraw Hill.
4. Wiseman, Enzyme Biotechnology, Ellis Horwood Pub.
Previous
Next

Comments

Post a Comment

Popular posts from this blog

EC8094 - SATELLITE COMMUNICATION (Syllabus) 2017-regulation Anna University

EC8094 - SATELLITE COMMUNICATION (Syllabus) 2017-regulation Anna University EC8094 SATELLITE COMMUNICATION  LPTC 3003 OBJECTIVES: • Understand the basics of satellite orbits • Understand the satellite segment and earth segment • Analyze the various methods of satellite access • Understand the applications of satellites • Understand the basics of satellite Networks UNIT I SATELLITE ORBITS       9 Kepler‟s Laws, Newton‟s law, orbital parameters, orbital perturbations, station keeping, geo stationary and non Geo-stationary orbits – Look Angle Determination- Limits of visibility – eclipse-Sub satellite point –Sun transit outage-Launching Procedures - launch vehicles and propulsion. UNIT II SPACE SEGMENT       9 Spacecraft Technology- Structure, Primary power, Attitude and Orbit control, Thermal control and Propulsion, communication Payload and supporting subsystems, Telemetry, Tracking and
Read more

HS8581 - PROFESSIONAL COMMUNICATION (Syllabus) 2017-regulation Anna University

HS8581 - PROFESSIONAL COMMUNICATION (Syllabus) 2017-regulation Anna University HS8581 PROFESSIONAL COMMUNICATION  LPTC 3003 OBJECTIVES: The course aims to: • Enhance the Employability and Career Skills of students • Orient the students towards grooming as a professional • Make them Employability Graduates • Develop their confidence and help them attend interviews successfully. UNIT I Introduction to Soft Skills-- Hard skills & soft skills - employability and career Skills—Grooming as a professional with values—Time Management—General awareness of Current Affairs UNIT II Self-Introduction-organizing the material - Introducing oneself to the audience – introducing the topic – answering questions – individual presentation practice–– presenting the visuals effectively – 5 minute presentations UNIT III Introduction to Group
Read more

OML751 - TESTING OF MATERIALS (Syllabus) 2017-regulation - Open Elective | Anna University

OML751 - TESTING OF MATERIALS (Syllabus) 2017-regulation - Open Elective | Anna University OML751 TESTING OF MATERIALS  LPTC 3003 OBJECTIVE: To understand the various destructive and non destructive testing methods of materials and its industrial applications. UNIT I INTRODUCTION TO MATERIALS TESTING 9 Overview of materials, Classification of material testing, Purpose of testing, Selection of material, Development of testing, Testing organizations and its committee, Testing standards, Result Analysis, Advantages of testing. UNIT II MECHANICAL TESTING 9 Introduction to mechanical testing, Hardness test (Vickers, Brinell, Rockwell), Tensile test, Impact test (Izod, Charpy) - Principles, Techniques, Methods, Advantages and Limitations, Applications. Bend test, Shear test, Creep and Fatigue test - Principles, Techniques, Methods, Advantages and Limitations, Applications.
Read more