BT3452 Syllabus - Industrial Enzymology - 2021 Regulation Anna University

BT3452 Syllabus - Industrial Enzymology - 2021 Regulation Anna University

BT3452

INDUSTRIAL ENZYMOLOGY

 LTPC

3003

COURSE OBJECTIVES:
To enable the students
• To learn enzyme reactions and its characteristics along with the production and purification process.
• To give the student a basic knowledge concerning biotransformation reactions with the usage of enzymes

UNIT I

INTRODUCTION TO ENZYMES

9

Classification of enzymes. Mechanisms of enzyme action; concept of active site and energetics of enzyme substrate complex formation; specificity of enzyme action; principles of catalysis – collision theory, transition state theory; role of entropy in catalysis.

UNIT II

KINETICS OF ENZYME ACTION

9

Kinetics of single substrate reactions; estimation of Michelis – Menten parameters, multisubstrate reactions - mechanisms and kinetics; turnover number; types of inhibition & models –substrate, product. Allosteric regulation of enzymes, Monod Changeux Wyman model, pH and temperature effect on enzymes & deactivation kinetics.


UNIT III

ENZYME IMMOBILIZATION AND BIOSENSORS

9

Physical and chemical techniques for enzyme immobilization – adsorption, matrix entrapment, encapsulation, cross-linking, covalent binding etc., - examples, advantages and disadvantages, design of enzyme electrodes and their application as biosensors in industry, healthcare and environment.

UNIT IV

PURIFICATION AND CHARACTERIZATION OF ENZYMES FROM NATURAL SOURCES

9

Production and purification of crude enzyme extracts from plant, animal and microbial sources; methods of characterization of enzymes; development of enzymatic assays

UNIT V

BIOTRANSFORMATION APPLICATIONS OF ENZYMES

9

Hydrolytic- Ester bond, Amide, Epoxides, Nitriles, Reduction reactions –aldehydes, Ketones, C=C, Oxidation reactions – Alkanes, Aromatic, Baeyer-Villiger, Enzymes in organic synthesis – esters, amide, peptide , Modified and Artificial Enzymes , Catalytic antibodies

TOTAL: 45 PERIODS

COURSE OUTCOMES:
1. The knowledge on enzyme and enzyme reactions will be the key step in to proceed towards various concepts in biotechnology.
2. The theoretical and practical aspects of kinetics will provide the importance and utility of enzyme kinetics towards research.
3. The process of immobilization has been increased steadily in food, pharmaceutical and chemical industries and thus this study will provide simple and easy method of implementation.
4. Ideas on Processing, Production and Purification of enzymes at an industrial scale will be helpful to work technologically.

TEXT BOOKS:
1. Trevor Palmer , Enzymes IInd Horwood Publishing Ltd
2. Faber K , Biotransformations in Organic Chemistry, IV edition , Springer

REFERENCES:
1. Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering, Marcel Dekker, Inc. 74
2. James M. Lee, Biochemical Engineering, PHI, USA.
3. James. E. Bailey & David F. Ollis, Biochemical Engineering Fundamentals, McGraw Hill.
4. Wiseman, Enzyme Biotechnology, Ellis Horwood Pub.

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