BT3452 Syllabus - Industrial Enzymology - 2021 Regulation Anna University
BT3452 Syllabus - Industrial Enzymology - 2021 Regulation Anna University
BT3452 | INDUSTRIAL ENZYMOLOGY | LTPC |
---|
3003
COURSE OBJECTIVES:
To enable the students
• To learn enzyme reactions and its characteristics along with the production and purification process.
• To give the student a basic knowledge concerning biotransformation reactions with the usage of enzymes
• To learn enzyme reactions and its characteristics along with the production and purification process.
• To give the student a basic knowledge concerning biotransformation reactions with the usage of enzymes
UNIT I | INTRODUCTION TO ENZYMES | 9 |
---|
Classification of enzymes. Mechanisms of enzyme action; concept of active
site and energetics of enzyme substrate complex formation; specificity of
enzyme action; principles of catalysis – collision theory, transition
state theory; role of entropy in catalysis.
UNIT II | KINETICS OF ENZYME ACTION | 9 |
---|
Kinetics of single substrate reactions; estimation of Michelis – Menten
parameters, multisubstrate reactions - mechanisms and kinetics; turnover
number; types of inhibition & models –substrate, product. Allosteric
regulation of enzymes, Monod Changeux Wyman model, pH and temperature
effect on enzymes & deactivation kinetics.
UNIT III | ENZYME IMMOBILIZATION AND BIOSENSORS | 9 |
---|
Physical and chemical techniques for enzyme immobilization – adsorption,
matrix entrapment, encapsulation, cross-linking, covalent binding etc., -
examples, advantages and disadvantages, design of enzyme electrodes and
their application as biosensors in industry, healthcare and environment.
UNIT IV | PURIFICATION AND CHARACTERIZATION OF ENZYMES FROM NATURAL SOURCES | 9 |
---|
Production and purification of crude enzyme extracts from plant, animal
and microbial sources; methods of characterization of enzymes; development
of enzymatic assays
UNIT V | BIOTRANSFORMATION APPLICATIONS OF ENZYMES | 9 |
---|
Hydrolytic- Ester bond, Amide, Epoxides, Nitriles, Reduction reactions
–aldehydes, Ketones, C=C, Oxidation reactions – Alkanes, Aromatic,
Baeyer-Villiger, Enzymes in organic synthesis – esters, amide, peptide ,
Modified and Artificial Enzymes , Catalytic antibodies
TOTAL: 45 PERIODS
COURSE OUTCOMES:
1. The knowledge on enzyme and enzyme reactions will be the key step in to
proceed towards various concepts in biotechnology.
2. The theoretical and practical aspects of kinetics will provide the importance and utility of enzyme kinetics towards research.
3. The process of immobilization has been increased steadily in food, pharmaceutical and chemical industries and thus this study will provide simple and easy method of implementation.
4. Ideas on Processing, Production and Purification of enzymes at an industrial scale will be helpful to work technologically.
2. The theoretical and practical aspects of kinetics will provide the importance and utility of enzyme kinetics towards research.
3. The process of immobilization has been increased steadily in food, pharmaceutical and chemical industries and thus this study will provide simple and easy method of implementation.
4. Ideas on Processing, Production and Purification of enzymes at an industrial scale will be helpful to work technologically.
TEXT BOOKS:
1. Trevor Palmer , Enzymes IInd Horwood Publishing Ltd
2. Faber K , Biotransformations in Organic Chemistry, IV edition , Springer
2. Faber K , Biotransformations in Organic Chemistry, IV edition , Springer
REFERENCES:
1. Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering, Marcel
Dekker, Inc. 74
2. James M. Lee, Biochemical Engineering, PHI, USA.
3. James. E. Bailey & David F. Ollis, Biochemical Engineering Fundamentals, McGraw Hill.
4. Wiseman, Enzyme Biotechnology, Ellis Horwood Pub.
2. James M. Lee, Biochemical Engineering, PHI, USA.
3. James. E. Bailey & David F. Ollis, Biochemical Engineering Fundamentals, McGraw Hill.
4. Wiseman, Enzyme Biotechnology, Ellis Horwood Pub.
Comments
Post a Comment