EL3304 Syllabus - Instrumental Methods Of Analysis - 2021 Regulation Anna University

EL3304 Syllabus - Instrumental Methods Of Analysis - 2021 Regulation Anna University

EL3304	INSTRUMENTAL METHODS OF ANALYSIS	LTPC

3003

COURSE OBJECTIVES:

• To impart knowledge on the principle and importance of various analytical instruments used for the characterization of various materials

UNIT I	INTRODUCTION TO SPECTROSCOPICAL METHODS OF ANALYSIS	9

Electromagnetic radiation: various ranges, dual properties, various energy levels, interaction of photons with matter, absorbance & transmittance and their relationship, permitted energy levels for the electrons of an atom and simple molecules, various electronic transitions in organic and inorganic compounds effected by UV, and visible radiations, various energy level diagrams of saturated, unsaturated and carbonyl compounds, excitation by UV and visible radiations, choice of solvents, cut off wavelengths for solvents

UNIT II	QUALITATIVE ANALYSIS BY UV AND VISIBLE SPECTROCOPY	9

Lamda max and epsilon max rules, Woodward -Fieser rules for the calculation of absorption maxima (Lamda max) for dienes and carbonyl compounds, Effects of auxochromes and effects of conjugation on the absorption maxima, Different shifts of absorption peaks(Bathochromic, hypsochromic, hypochromic), Instrumentation for UV and Visible spectrophotometers (source, optical parts and detectors), Applications of UV and Visible spectroscopy.

UNIT III	QUANTITATIVEANALYSIS BY UV AND VISIBLE SPECTROCOPY	9

Beer-Lambert's law, limitations, deviations (real, chemical, instrumental), estimation of inorganic ions such as Fe, Ni and estimation of nitrite using Beer -Lambert's law, multicomponent analysis (no overlap, single way overlap and two way overlap), photometric titration(experimental set -up and various types of titrations and their corresponding curves).

UNIT IV	IR SPECTROSCOPY	9

Theory of IR spectroscopy, various stretching and vibration modes for diatomic and triatomic molecules (both linear and nonlinear), various ranges of IR (near, mid, finger print and far) and their usefulness, Instrumentation (only the sources and detectors used in different regions), sample preparation techniques, qualitative analysis of alkanes, alkenes and carbonyl compounds.

UNIT V	CHROMATOGRAPHIC METHODS	9

Classification of chromatographic methods, column, thin layer, paper, gas, High Performance Liquid Chromatographical methods (principle, mode of separation and technique).

TOTAL: 45 PERIODS

COURSE OUTCOMES: At the end of this course, the students would

1. Have thorough understanding of theory, instrumentation and applications of analytical equipments used in industries for testing the quality of raw materials, intermediates and finished products.
2. Know the importance of analytical instrumentation during the purification, compounding and formulating the finished product.

TEXT BOOKS:

1. Sivasankar B., “Instrumental Methods of Analysis”, Oxford University Press, 2012. 2. William Kemp, Organic Spectroscopy, 3rd Edition, Palgrave publishers, 2007.

REFERENCES:

1. Douglas A. Skoog, F. James Holler, Stanley R. Crouch, Instrumental Analysis, CENGAGE Learning, India, 7th Edition, 2007.
2. Willard H.H, Merritt L.L, Dean J.A and Settle F.A, Instrumental method of analysis, 7th edition, Wadsworth Publishing Company, 1988.
3. Gurdeep R. Chatwal, Sharma K. Anand, Instrumental methods of Chemical Analysis, Himalaya Publishers, New Delhi, 2014.
4. John R Dyer, Applications of Absorption Spectroscopy of Organic Compounds, Prentice-hall of India Pvt. Ltd., 2012.
5. Robert M. Silverstein, Francis X. Webstrer, David Kiemle, David L. Bryce, Spectrometric Identification of Organic Compounds, Wiley, 8th Edition, 2010.