OBT354 Syllabus - Fundamentals Of Cell And Molecular Biology - 2021 Regulation - Open Elective | Anna University

OBT354 Syllabus - Fundamentals Of Cell And Molecular Biology - 2021 Regulation - Open Elective | Anna University

OBT354

FUNDAMENTALS OF CELL AND MOLECULAR BIOLOGY

 L T P C
3003

OBJECTIVES:
• To provide knowledge on the fundamentals of cell biology.
• To understand the signalling mechanisms.
• Understand basic principles of molecular biology at intracellular level to regulate growth, division and development.

UNIT I

INTRODUCTION TO CELL

9
Cell, cell wall and Extracellular Matrix (ECM), composition, cellular dimensions, Evolution, Organisation, differentiation of prokaryotic and Eukaryotic cells, Virus, bacteria,cyanobacteria, mycoplasma and prions.

UNIT II

CELL ORGANELLES

9
Molecular organisation, biogenesis and functin Mitochondria, endoplasmic reticulam, golgi apparatus, plastids, chloroplast, leucoplast, centrosome, lysosome, ribosome, peroxisome, Nucleus and nucleolus. Endo membrane system, concept of compartmentalisation.


UNIT III

BIO-MEMBRANE TRANSPORT

9
Physiochemical properties of cell membranes. Molecular constitute of membranes, asymmetrical organisation of lipids and proteins. Solute transport across membrane’s-fick’s law, simple diffusion, passive-facilitated diffusion, active transport- primary and secondary, group translocation, transport ATPases, membrane transport in bacteria and animals. Transportmechanism- mobile carriers and pores mechanisms. Transport by vesicle formation, endocytosis, exocytosis, cell respiration.

UNIT IV

CELL CYCLE

9
Cell cycle- Cell division by mitosis and meosis, Comparision of meosis and mitosis, regulation of cell cycle, cell lysis, Cytokinesis, Cell signaling, Cell communication, Cell adhesion and Cell junction, cell cycle checkpoints.

UNIT V

CENTRAL DOGMA

9
Overview of Central dogma DNA replication: Meselson & Stahl experiment, bi–directional DNA replication, Okazaki fragments. Structure and function of mRNA, rRNA and tRNA. RNA synthesis: Initiation, elongation and termination of RNA synthesis Introduction to Genetic code- Steps in translation: Initiation, Elongation and termination of protein synthesis.

TOTAL: 45 PERIODS

OUTCOMES:
• Understanding of cell at structural and functional level.
• Understand the central dogma of life and its significance.
• Comprehend the basic mechanisms of cell division.

TEXT BOOKS:
1. Cooper, G.M. and R.E. Hansman “The Cell: A Molecular Approach”, 8th Edition, Oxford University Press, 2018
2. Friefelder, David. “Molecular Biology.” Narosa Publications, 1999
3. Weaver, Robert F. “Molecular Biology” IInd Edition, Tata McGraw-Hill, 2003.

REFERENCES:
1. Lodish H, Berk A, MatsudairaP,Kaiser CA, Krieger M, Schot MP, Zipursky L, Darnell J. Molecular Cell Biology, 6th Edition, 2007.
2. Becker, W.M. etal., “The World of the Cell”, 9th Edition, Pearson Education, 2003.
3. Campbell, N.A., J.B. Recee and E.J. Simon “Essential Biology”, VIIrd Edition, Pearson International, 2007.
4. Alberts, Bruce etal., “Essential Cell Biology”, 4th Edition, W.W. Norton, 2013.
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