BT8020 - BIOCONJUGATE TECHNOLOGY AND APPLICATIONS (Syllabus) 2017-regulation Anna University

BT8020 - BIOCONJUGATE TECHNOLOGY AND APPLICATIONS (Syllabus) 2017-regulation Anna University

BT8020	BIOCONJUGATE TECHNOLOGY AND APPLICATIONS	LPTC

3003

OBJECTIVES:

To enable the students • To understand the functional targets and chemistry of active groups. • To gain knowledge about the linkers and cleavable reagent systems. • To know about enzyme, nucleic acid modification and its application in bioconjugation

UNIT I	FUNCTIONAL TARGETS	9

Modification of Amino Acids, Peptides and Proteins – Modification of sugars, polysaccharides and glycoconjugates – modification of nucleic acids and oligonucleotides.

UNIT II	CHEMISTRY OF ACTIVE GROUPS	9

Amine reactive chemical reactions – Thiol reactive chemical reactions – carboxylate reactive chemical reactions – hydroxyl reactive chemical reactions – aldehyde and ketone reactive chemical reactions – Photoreactive chemical reactions.

UNIT III	BIOCONJUGATE REAGENTS	9

Zero length cross linkers – Homobifunctional cross linkers – Heterobifunctional cross linkers – Trifunctional cross linkers – Cleavable reagent systems – tags and probes.

UNIT IV	ENZYME AND NUCLEIC ACID MODIFICATION AND CONJUGATION	9

Properties of common enzymes – Activated enzymes for conjugation – biotinylated enzymes – chemical modification of nucleic acids – biotin labeling of DNA- enzyme conjugation to DNA – Fluorescent of DNA.

UNIT V	BIOCONJUGATE APPLICATIONS	9

Preparation of Hapten-carrier Immunogen conjugates - antibody modification and conjugation immunotoxin conjugation techniques – liposome conjugated and derivatives- Colloidal – gold- labeled proteins – modification with synthetic polymers.

TOTAL : 45 PERIODS

OUTCOMES: Upon completion of this course, the student would know about

• Joining of two molecules to form a hybrid conjugate with the help of linkers.
• Active groups of various chemical reactions and targets of the functional groups.
• Antibody modification and conjugation.

REFERENCE:

1. Bioconjugate Techniques, G.T. Hermanson, Academic Press, 1999.