BT8004 - ADVANCED BIOCHEMISTRY (Syllabus) 2017-regulation Anna University

BT8004 - ADVANCED BIOCHEMISTRY (Syllabus) 2017-regulation Anna University

BT8004	ADVANCED BIOCHEMISTRY	LPTC

3003

OBJECTIVES:

• To orient towards the application of knowledge acquired in solving clinical problems. • To provide a base for molecular modelling and drug designing

UNIT I	METABOLISM OF AMINO ACIDS	9

Biosynthesis of Gly, Ser and Cys; Biosynthesis of six essential amino acids (Met, Thr, Lys, Ile,Val, Leu) and regulation of branched chain amino acids (concerted inhibition, allosteric regulation and enzyme multiplicity, sequential feedback) from oxaloacetate and pyruvate; Biosynthesis of aromatic amino acids. Metabolic disorders associated with branched chain and aromatic amino acid degradation. Important molecules derived from amino acids (auxins, DOPA, Serotonin, porphyrins, T3, T4, Adrenaline, Noradrenaline, histamine, GABA, polyamines etc)

UNIT II	PROTEIN TRANSPORT AND DEGRADATION	9

Protein targeting, signal sequence, secretion; Folding, Chaperons and targeting of organelle proteins, Protein degradation, receptor-mediated endocytosis, turnover.

UNIT III	BIOCHEMISTRY OF MUSCLE CONTRACTION	9

Contractile proteins, Actin, Myosin, Actin Polymerization, acto-myosin complexes, mechanism of myosin ATPase activity, excitation – contraction coupling nad relaxation, microtubules, microfilaments and their role in organelle movements.

UNIT IV	VITAMINS AND COENZYMES	9

Fat Soluble Vitamins, provitamins (A, D, E and K). Structure,physiological significance and deficiency symptoms. Water soluble vitamins, structure, coenzyme role and deficiency symptoms. Thiamine, riboflavin, pyridoxine, niacin, folic acid, biotin and Vitamin B12. Recommended dietary intake. Coenzymes: Their role in metabolic pathways.NAD, FAD,TPP, PLP, carboxy biotin

UNIT V	HORMONES	9

Introduction. Effects of Hormones. Chemical classification of hormones.Peptide hormonevasopressin,protein hormone- insulin. Lipid and phospholipid derived hormonesprostaglandin and phospholipids. Steroid hormones-testosterone, estrogen, cortisol. Monoamines: thyroxine, adrenaline. Mechanism of action of the different classes of hormones.

TOTAL : 45 PERIODS

OUTCOMES: Upon completion of advanced biochemistry, students will be able

• To recognize how fundamental chemical principles and reactions are utilized in biochemical Processes.
• To apply knowledge gained in food and drug industries.
• To define various metabolic concepts for applying them to solve clinical problems.
• To summarize the knowledge of biomolecules to use them in biotechnology industry

TEXT BOOKS:

1. Nelson, D.L et al., “Lehninger’s Principles of Biochemistry” Stryer, Lubert. “Biochemistry”.IVth Edition, W.H Freeman & Co., 2000.
2. Voet, D.J and J.G. Voet and C.W. Pratt “Principles of Biochemistry” IIIrd Edition, JohnWiley & Sons Inc., 2008.
3. Murray, R.K., et al., “Harper’s Illustrated Biochemistry”. XXVIIth Edition. McGraw-Hill,2006.

REFERENCES:

1. Creighton. T.E., “Proteins: Structure and Molecular Properties” IInd Edition, W.H. Freeman and Co.,1993.
2. Salway, J.G., “Metabolism at a Glance”. IInd Edition, Blackwell Science Ltd., 2000.