BT8404 - BIOPROCESS PRINCIPLES (Syllabus) 2017-regulation Anna University

BT8404 - BIOPROCESS PRINCIPLES (Syllabus) 2017-regulation Anna University

BT8404	BIOPROCESS PRINCIPLES	LPTC

3003

OBJECTIVES:

• To impart knowledge on design and operation of fermentation processes with all its prerequisites.
• To endow the students with the basics of microbial kinetics, metabolic stoichiometry and energetics.

UNIT I	OVERVIEW OF FERMENTATION PROCESSES	9

Overview of fermentation industry, general requirements of fermentation processes, basic configuration of fermentor (CSTR) and ancillaries, main parameters to be monitored and controlled in fermentation processes.

UNIT II	RAW MATERIALS AND MEDIA DESIGN FOR FERMENTATION PROCESS	9

Criteria for good medium, medium requirements for fermentation processes, carbon, nitrogen, minerals, vitamins and other complex nutrients, oxygen requirements, medium formulation of optimal growth and product formation, examples of simple and complex media, design of various commercial media for industrial fermentations – medium optimization methods

UNIT III	STERILIZATION KINETICS	9

Thermal death kinetics of microorganisms, batch and continuous heat sterilization of liquid media, filter sterilization of liquid media, air sterilization and design of sterilization equipment - batch and continuous.

UNIT IV	METABOLIC STOICHIOMETRY AND ENERGETICS	9

Stoichiometry of cell growth and product formation, elemental balances, degrees of reduction of substrate and biomass, available electron balances, yield coefficients of biomass and product formation, maintenance coefficients energetic analysis of microbial growth and product formation, oxygen consumption and heat evolution in aerobic cultures, thermodynamic efficiency of growth.

UNIT V	KINETICS OF MICROBIAL GROWTH AND PRODUCT FORMATION	9

Batch cultivation and continuous cultivation. Simple unstructured models for microbial growth, Monod model, growth of filamentous organisms, product formation kinetics - Leudeking- Piret models, substrate and product inhibition on cell growth and product formation. Biomass estimation – Direct and Indirect methods.

TOTAL : 45 PERIODS

OUTCOMES: Upon completion of the course in Bioprocess Principles graduates will be able to

• Apply engineering principles to systems containing biological catalysts to meet the needs of the society.
• Convert the promises of molecular biology and genetic engineering into new processes to make bio-products in economically feasible way.

TEXT BOOKS:

1. Shuler, Michael L. and Fikret Kargi, “ Bioprocess Engineering “, Prentice Hall, 1992.
2. Doran, Pauline “of Bioprocess Engineering Principles “. Elsevier, 1995

REFERENCES:

1. Lydersen, Bjorn K. “Bioprocess Engineering Systems, Equipment and Facilities” John Wiley, 1994.
2. Bailey, James E. and David F. Ollis, “ Biochemical Engineering Fundamentals”, IInd Edition. McGraw Hill , 1986.
3. Peter F. Stanbury, Stephen J. Hall & A. Whitaker, Principles of Fermentation Technology, Science & Technology Books.
4. Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering, Marcel Dekker, Inc.