BT8751 - DOWNSTREAM PROCESSING (Syllabus) 2017-regulation Anna University

BT8751 - DOWNSTREAM PROCESSING (Syllabus) 2017-regulation Anna University

BT8751

DOWNSTREAM PROCESSING

 LPTC
3003

OBJECTIVES: To enable the students to
• Understand the methods to obtain pure proteins, enzymes and in general about product development R & D.
• Have depth knowledge and hands on experience with on Downstream processes required in multi-factorial manufacturing environment in a structured and logical fashion

UNIT I

INTRODUCTION

9
Introduction to downstream processing, principles, characteristics of bio-molecules and bioprocesses. Cell disruption for product release – mechanical, enzymatic and chemical methods. Pre treatment and stabilisation of bio-products.

UNIT II

PHYSICAL METHODS OF SEPARATION

9
Unit operations for solid-liquid separation - filtration and centrifugation.


UNIT III

ISOLATION OF PRODUCTS

9
Adsorption, liquid-liquid extraction, aqueous two-phase extraction, membrane separation – ultrafiltration and reverse osmosis, dialysis, precipitation of proteins by different methods.

UNIT IV

PRODUCT PURIFICATION

9
Chromatography – principles, instruments and practice, adsorption, reverse phase, ion exchange, size exclusion, hydrophobic interaction, bio-affinity and pseudo affinity chromatographic techniques.

UNIT V

FINAL PRODUCT FORMULATION AND FINISHING OPERATIONS

9
Crystallization, drying and lyophilization in final product formulation.

TOTAL : 45 PERIODS

OUTCOMES: Upon success completion of this course, the students will be able to:
• Define the fundamentals of downstream processing for product recovery
• Understand the requirements for successful operations of downstream processing
• Describe the components of downstream equipment and explain the purpose of each
• Apply principles of various unit operations used in downstream processing and enhance problem solving techniques

TEXT BOOKS:
1. Belter, P.A., E.L. Cussler and Wei-Houhu “Bioseparations – Downstream Processing for Biotechnology”, John Wiley, 1988.
2. Sivasankar, B. “Bioseparations: Principles and Techniques”. PHI, 2005.
3. Asenjo, Juan A. “Separation Processes in Biotechnology”. CRC / Taylor & Francis, 1990.

REFERENCES:
1. Ghosh, Raja “Principles of Bioseparations Engineering”. World Scientific, 2006
2. “Product Recovery in Bioprocess Technology”. (BIOTOL – Biotechnology by Open Learning Series). Butterworth – Heinmann / Elsevier, 2004.
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